FastQCFastQC Report
Thu 26 May 2016
SRR1512685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875822
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67020.35728336697191954No Hit
GTCCTACAGTGGACATTTCTAAATT44200.23563003312681052No Hit
GGTATCAACGCAGAGTACTTTTTTT43050.2294993874685338No Hit
GTCCTAAAGTGTGTATTTCTCATTT42150.22470149086640417No Hit
TATCAACGCAGAGTACTTTTTTTTT39640.21132069034268708No Hit
CTGTAGGACGTGGAATATGGCAAGA39590.21105414053145766No Hit
CTTTAGGACGTGAAATATGGCGAGG33660.17944133291964803No Hit
GTCCTACAGTGTGCATTTCTCATTT25610.1365268133117108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7600.013.2322884
GGTATCA14950.012.1460161
GTCCTAG2450.011.6181021
ATAGGAC2900.011.4501913
CTAGCAC1354.829417E-911.2442093
TGTAGGA26850.011.2010232
TAGGACT3150.011.1438134
GTAGGAC26150.011.0654953
GACGTGG14100.011.0348767
TAGGACA7700.010.965844
GCGAATC700.001504335610.84612115
CCTACCG700.001508380210.842633
GTATTAA2001.8189894E-1210.7968231
GGCGAGG11150.010.79400619
ATCCTAC2550.010.7775771
AGGACGT26850.010.7770095
GGACGTG26150.010.7389726
GTCCTAA14350.010.7386841
TGGACCG803.8183347E-410.6732145
CTGTAGG24100.010.5891141
Produced by FastQC (version 0.11.5)