FastQCFastQC Report
Thu 26 May 2016
SRR1512685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875822
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84860.4523883396185779No Hit
TATCAACGCAGAGTACTTTTTTTTT59610.31778068494771894No Hit
GGTATCAACGCAGAGTACTTTTTTT58610.31244968872313045No Hit
CTGTAGGACGTGGAATATGGCAAGA47370.252529291158756No Hit
GTCCTACAGTGGACATTTCTAAATT45030.24005475999321896No Hit
GTCCTAAAGTGTGTATTTCTCATTT44630.23792236150338356No Hit
CTTTAGGACGTGAAATATGGCGAGG39690.21158724015391653No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27290.14548288696901945No Hit
GTCCTACAGTGTGCATTTCTCATTT26550.141537949762824No Hit
CTGAAGGACCTGGAATATGGCGAGA22780.12144009399612543No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21800.11621571769602872No Hit
CTGTAGGACCTGGAATATGGCGAGA20060.10693978426524478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG655.418781E-513.1628445
TAGGACC7650.012.7989174
GACCGGG604.0967506E-412.6651917
GGTATCA18750.011.7743351
GGCGAGG10100.011.563333519
GCACCGT855.3245196E-511.176366
CTGTGCG855.3303083E-511.1751699
ACCGAGC600.005881523211.0820428
GTCTTAG2200.010.8135181
TCGAACT1502.1518645E-910.76110719
CTGTAGG27650.010.5999841
TGTAGGA28200.010.5515392
TAGACAG2750.010.3707265
TTAGACA1855.820766E-1110.2767484
AGGACCT15750.010.26098255
GTATTAG1409.454925E-810.1956021
CGTGCGC750.002650568310.13215310
GTAGGAC28250.010.0948583
CTCGAAC1701.5734258E-910.0536318
GTATTAA1803.8198777E-1010.0445571
Produced by FastQC (version 0.11.5)