FastQCFastQC Report
Thu 26 May 2016
SRR1512684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497737
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60910.24386074274433217No Hit
GTCCTACAGTGGACATTTCTAAATT41300.16534967452538038No Hit
GTCCTAAAGTGTGTATTTCTCATTT39030.15626144786260524No Hit
GGTATCAACGCAGAGTACTTTTTTT36420.14581198901245407No Hit
TATCAACGCAGAGTACTTTTTTTTT34440.13788481333302907No Hit
CTGTAGGACGTGGAATATGGCAAGA32840.13147901480420077No Hit
CTTTAGGACGTGAAATATGGCGAGG31570.1263944122219433No Hit
GTCCTACAGTGTGCATTTCTCATTT30380.12163009956612726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGT456.854707E-414.75193311
ATTGCGA655.5046665E-513.14115617
TATTGCG655.505707E-513.14089216
TAGGACC9300.013.0584464
CGACTGT550.003099068812.0753536
TCCAACG2300.011.53547118
GACATCG600.005933922211.0690747
CTTATAC1803.6379788E-1211.06885053
TGTAGGA27800.010.8524882
AGACCGT700.001508054810.8431746
GTAGGAC25700.010.7796693
TGGCGAG24500.010.365108518
GGACCGT1201.5499936E-610.2784256
GTCCTAC23600.010.2710561
GTACTAG1156.9765483E-610.2403211
AGGACCT18750.010.17089755
ACCGTAT750.002747506410.0886048
CTGTAGG24400.010.0549591
GATATAC6350.010.0454581
TCCTACA27900.010.0314752
Produced by FastQC (version 0.11.5)