FastQCFastQC Report
Thu 26 May 2016
SRR1512684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497737
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80500.32229173848167364No Hit
TATCAACGCAGAGTACTTTTTTTTT53110.21263247491629422No Hit
GGTATCAACGCAGAGTACTTTTTTT51150.20478537171847955No Hit
GTCCTAAAGTGTGTATTTCTCATTT42670.1708346395156896No Hit
GTCCTACAGTGGACATTTCTAAATT42050.16835239258576865No Hit
CTGTAGGACGTGGAATATGGCAAGA40740.16310764504029046No Hit
CTTTAGGACGTGAAATATGGCGAGG37550.15033608422343905No Hit
GTCCTACAGTGTGCATTTCTCATTT31280.12523336123859316No Hit
GTACATGGGAAGCAGTGGTATCAAC29640.11866741774654416No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25620.10257284894286307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8700.012.8922484
CCAACGA1457.2759576E-1212.44175719
CGACTAA1459.822543E-1111.78716417
GGCGAGG11850.011.61838519
TACACCG855.2922132E-511.1833785
TGTAGGA27100.010.8743112
CGCGCGC700.001493736510.85558710
TTAGGAC16650.010.7327123
AGCGACT1605.493348E-1010.68254515
GCGACTA1605.493348E-1010.68233116
TGGCGAG24500.010.58038318
GTAGGAC26500.010.5455033
AATGTCC6400.010.5375518
AGGACGT24850.010.5195765
GTTTAGA2001.8189894E-1210.46694851
GTATTAC1659.0403773E-1010.3804451
GGACGTG24400.010.2395166
AGTCGTC1409.715768E-810.1771138
AGGACCT15050.010.1690725
ATGGCGA25900.010.15511417
Produced by FastQC (version 0.11.5)