FastQCFastQC Report
Thu 26 May 2016
SRR1512683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212059
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63480.2869724541705262No Hit
GGTATCAACGCAGAGTACTTTTTTT39970.180691383005607No Hit
GTCCTACAGTGGACATTTCTAAATT37390.16902804129546273No Hit
TATCAACGCAGAGTACTTTTTTTTT35910.16233744217491486No Hit
GTCCTAAAGTGTGTATTTCTCATTT34530.1560989105625121No Hit
CTGTAGGACGTGGAATATGGCAAGA32430.14660549289146446No Hit
CTTTAGGACGTGAAATATGGCGAGG26860.12142533268778093No Hit
GTACATGGGAAGCAGTGGTATCAAC25590.11568407533433783No Hit
GTCCTACAGTGTGCATTTCTCATTT22690.10257411759812915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7400.013.45972254
GTAGGAC21350.010.9298893
GTCCTAA10350.010.9129181
ACTGCGT700.00154807910.8090718
TGTAGGA22150.010.7920842
GTGTAGG4800.010.4354771
TGGCGAG20000.010.32952918
TAGAAAT5150.010.3147644
AGGACGT21250.010.3117315
GTATAAA3350.010.26141
ATAGGAC2500.010.2447723
GTATAGA2500.010.2144921
TAGGACA6600.010.2045284
GGCGAGG9850.010.201447519
AAGATCG750.002681147310.1182935
GGACGTG21050.010.0942586
TTACACC2350.010.0913834
GACGTGA11700.010.0534327
AGGACCT15100.010.0512845
GTCCTAC19250.010.0512231
Produced by FastQC (version 0.11.5)