FastQCFastQC Report
Thu 26 May 2016
SRR1512683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212059
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72720.32874349192313584No Hit
TATCAACGCAGAGTACTTTTTTTTT48450.2190267076963137No Hit
GGTATCAACGCAGAGTACTTTTTTT48230.2180321591783944No Hit
GTCCTACAGTGGACATTTCTAAATT39210.17725566994370404No Hit
CTGTAGGACGTGGAATATGGCAAGA39130.17689401593718793No Hit
GTCCTAAAGTGTGTATTTCTCATTT35150.1589017291130119No Hit
CTTTAGGACGTGAAATATGGCGAGG33130.14976996544848034No Hit
GTACATGGGAAGCAGTGGTATCAAC27450.12409253098583717No Hit
GTCCTACAGTGTGCATTTCTCATTT25320.11446349306234599No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22710.10266453109975819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7400.013.4875154
ATCGCCG500.001505907713.29294419
GGACCGC701.0918724E-412.2136036
ACCGGGG658.057005E-411.68689615
CGCCCTA1604.5474735E-1111.27551816
AATCGCC600.005899258411.077702518
CGTGCGC700.00149337510.855810
TACTAGC909.443248E-510.5638042
TGTAGGA23200.010.449972
CTGTAGG23200.010.2937371
TAGGACA6250.010.1898824
GTAGGAC22450.010.1617693
GTCGAAA750.002658517810.12864415
GGCGAGG9700.010.08227719
GGTATCA21050.09.9891261
AGGACCT16200.09.975125
TAGAAAT6650.09.8628374
GTCCTAA10350.09.8362771
GAAATGT5850.09.7430456
AATGTCC6050.09.7343328
Produced by FastQC (version 0.11.5)