FastQCFastQC Report
Thu 26 May 2016
SRR1512682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987587
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78420.26248607990327977No Hit
GTCCTAAAGTGTGTATTTCTCATTT70850.23714790565094843No Hit
CTGTAGGACGTGGAATATGGCAAGA66690.22322362495217712No Hit
GTATCAACGCAGAGTACTTTTTTTT63190.21150848494119168No Hit
CTTTAGGACGTGAAATATGGCGAGG54410.1821202194279196No Hit
GTCCTACAGTGTGCATTTCTCATTT51040.17084021318877074No Hit
GGTATCAACGCAGAGTACTTTTTTT38790.12983722315032165No Hit
TATCAACGCAGAGTACTTTTTTTTT37210.12454867423107678No Hit
CTGTAGGACCTGGAATATGGCGAGA34030.11390463273538143No Hit
GTACATGGGAAGCAGTGGTATCAAC33530.11223104130524066No Hit
CTGAAGGACCTGGAATATGGCGAGA30060.10061631678006365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14600.013.2560454
CGTGCGC1001.979346E-611.3767510
TTACGGA600.005937213511.0683644
AGGACCT27550.010.9851455
TGTAGGA45350.010.9199982
GTAGGAC44950.010.8272223
GGACCTG28000.010.6730666
GGCGAGG18350.010.64148819
GACGTGG23000.010.6008827
GTCTACG750.002013278710.4735761
GTGTAGG5250.010.4735761
ATTTCTA28500.010.45693315
GTCCTAC43200.010.4326631
GTCCTAA20500.010.3937461
CATTTCT55900.010.39104314
AATGTCC10650.010.3896378
GGACGTG45400.010.3857356
AGGACGT46450.010.3756345
CGACTAA1659.458745E-1010.35417317
CTGTAGG42400.010.351611
Produced by FastQC (version 0.11.5)