FastQCFastQC Report
Thu 26 May 2016
SRR1512682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987587
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA84180.2817658531785016No Hit
GTCCTACAGTGGACATTTCTAAATT81730.27356525517081176No Hit
GTATCAACGCAGAGTACTTTTTTTT74980.25097177086391126No Hit
GTCCTAAAGTGTGTATTTCTCATTT73790.24698862326017618No Hit
CTTTAGGACGTGAAATATGGCGAGG69880.2339011382764753No Hit
GTCCTACAGTGTGCATTTCTCATTT54040.1808817617696154No Hit
GGTATCAACGCAGAGTACTTTTTTT50290.1683298260435596No Hit
TATCAACGCAGAGTACTTTTTTTTT49080.16427973478261887No Hit
CTGTAGGACCTGGAATATGGCGAGA41110.13760268738617487No Hit
CTGAAGGACCTGGAATATGGCGAGA39750.13305051869619194No Hit
GTACATGGGAAGCAGTGGTATCAAC38070.1274272514909189No Hit
ATTTAGAAATGTCCACTGTAGGACG30700.10275851381064383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT250.006040727318.99598112
TAGGACC15200.013.8834544
TCCAACG2050.012.04280518
GGCGAGG17150.011.23913319
ACCGTGC1451.2223609E-911.1365088
AGGACCT31800.011.0906445
GATATAC6400.011.0024981
GGACCTG30350.010.9548526
AATGTCC12850.010.8663168
CGGGCCG700.001483913510.8643055
CCGTCCA803.7773265E-410.6861349
TGCGCAT803.7773265E-410.68613410
CCAACGA1605.493348E-1010.6820219
ATTTAGA12900.010.5483831
TGTAGGA45500.010.4884082
TAGAAAT13650.010.4459224
GTCCTAA23600.010.4430491
TGGCGAG44800.010.27949818
GAAATGT13600.010.2677586
CTGTAGG44450.010.2328281
Produced by FastQC (version 0.11.5)