FastQCFastQC Report
Thu 26 May 2016
SRR1512681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63230.24548180317119161No Hit
GTATCAACGCAGAGTACTTTTTTTT63100.24497709600035097No Hit
CTGTAGGACGTGGAATATGGCAAGA55120.2139958404364397No Hit
GTCCTAAAGTGTGTATTTCTCATTT54860.21298642609475837No Hit
CTTTAGGACGTGAAATATGGCGAGG43560.1691157258601472No Hit
GGTATCAACGCAGAGTACTTTTTTT37680.1462874322867389No Hit
TATCAACGCAGAGTACTTTTTTTTT36660.142327422177066No Hit
GTCCTACAGTGTGCATTTCTCATTT33900.13161210070383356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG400.005326915514.2311484
TAGGACC10250.013.6063664
GTCCTAT3050.011.9168921
CGTGCGC855.4368236E-511.15384210
ATTAGAC1303.3325705E-810.947253
GGCGAGG15300.010.84147319
GTGTAGG5000.010.8057221
TAGGACT4100.010.6444364
GTCCTAG3050.010.6285791
AGGACCT22850.010.50468355
TGTAGGA35800.010.4416792
GTCCTAA16800.010.408111
GTCCTAC31250.010.3734931
TGGCGAG33500.010.32394618
TCCTACA36050.010.3166312
ATCCTAC3950.010.19642451
GTAGGAC35400.010.1576463
ATTTCTA22900.010.07000615
TTAGGAC20500.010.042993
GGACCTG22500.010.0355946
Produced by FastQC (version 0.11.5)