FastQCFastQC Report
Thu 26 May 2016
SRR1512681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575751
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76520.2970784054825175No Hit
CTGTAGGACGTGGAATATGGCAAGA66250.2572065389861054No Hit
GTCCTACAGTGGACATTTCTAAATT64200.2492476951382335No Hit
GTCCTAAAGTGTGTATTTCTCATTT57280.2223817441980999No Hit
GGTATCAACGCAGAGTACTTTTTTT53900.20925935775624274No Hit
TATCAACGCAGAGTACTTTTTTTTT52970.20564876030330573No Hit
CTTTAGGACGTGAAATATGGCGAGG52590.20417346241930995No Hit
GTCCTACAGTGTGCATTTCTCATTT34770.13498975638561336No Hit
CTGAAGGACCTGGAATATGGCGAGA31130.12085795560207489No Hit
CTGTAGGACCTGGAATATGGCGAGA27080.10513438604896203No Hit
GTACATGGGAAGCAGTGGTATCAAC26100.10132967045339397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG551.9510988E-413.826385
TAGGACC10100.013.3640994
GAACAGT4500.011.399016
CCGCGCG600.00588378711.0817259
AGGACCT21100.010.9472485
GGCGAGG15550.010.93049519
GTCCTAT2700.010.9266191
CGCTCAC803.7775506E-410.68594810
TAGAAAT9900.010.6576264
GGACGTG35000.010.5847956
AGGACGT35500.010.5766935
CCCCGTA909.5656986E-510.55054115
GGACCTG22000.010.5354486
ATTTAGA10250.010.4916171
GCGTACA1451.4279067E-810.4906082
GCCGGTT1002.4038993E-510.44848311
AAATGTC9500.010.3984917
GGTATCA21450.010.3818881
AATGTCC10200.010.3367358
AGCGTAC1201.4906491E-610.30979251
Produced by FastQC (version 0.11.5)