FastQCFastQC Report
Thu 26 May 2016
SRR1512680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60120.24905434330973006No Hit
GTCCTACAGTGGACATTTCTAAATT51160.21193646380116082No Hit
GTCCTAAAGTGTGTATTTCTCATTT49230.20394120627308734No Hit
CTGTAGGACGTGGAATATGGCAAGA43540.18036969573695355No Hit
CTTTAGGACGTGAAATATGGCGAGG39040.16172790357305158No Hit
GGTATCAACGCAGAGTACTTTTTTT37030.15340123640650874No Hit
TATCAACGCAGAGTACTTTTTTTTT35150.14561310990247858No Hit
GTCCTACAGTGTGCATTTCTCATTT30920.12808982526841073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9700.012.4206384
CCGCATA550.003163795212.0420399
GGTATCA18200.011.0025381
GTACTAG906.828552E-510.9066541
GGCGAGG14150.010.85107219
TTAGGAC19250.010.7928053
GTATACG650.00835323810.5710651
GTCCTAA15700.010.5662561
GTGTAGG4400.010.485261
TTTAGAA10400.010.3990212
AGGACCT20850.010.3738685
TAGAAAT9000.010.2244774
GTACCGA750.00267959210.1190716
GGACGTG31400.010.0908746
AGGACGT32350.010.08779055
GTCGAGG1804.110916E-1010.00449619
GTCTAGA2950.09.9823621
GTCCTAC29600.09.9817981
TTTAGGA21950.09.9406382
TGTAGGA31750.09.8603232
Produced by FastQC (version 0.11.5)