FastQCFastQC Report
Thu 26 May 2016
SRR1512680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74850.31007514299290245No Hit
CTGTAGGACGTGGAATATGGCAAGA54650.22639420927938703No Hit
GTCCTAAAGTGTGTATTTCTCATTT52450.21728044422147938No Hit
GTCCTACAGTGGACATTTCTAAATT52000.21541626500508923No Hit
TATCAACGCAGAGTACTTTTTTTTT50260.20820810536838047No Hit
GGTATCAACGCAGAGTACTTTTTTT50010.20717245024816366No Hit
CTTTAGGACGTGAAATATGGCGAGG49390.2046040255500261No Hit
GTCCTACAGTGTGCATTTCTCATTT33000.1367064758686143No Hit
CTGAAGGACCTGGAATATGGCGAGA28000.11599337346427882No Hit
CTGTAGGACCTGGAATATGGCGAGA26090.10808096834582265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGG802.0040425E-613.0605627
TGTCGAG1457.2759576E-1212.44256318
GGCGAAA550.003079861212.0853619
TAGGACC11550.011.932554
TATATCG657.990254E-411.6985735
CGAACCG1001.9347735E-611.39807412
GGTATCA17900.011.3773111
GGCGAGG14200.010.96679519
TATGTCG1658.185452E-1110.93437416
CTTATAC2350.010.9205163
TCCTATA3400.010.9038022
TGTAGGA34200.010.8400362
CCACCTT9200.010.83966113
CACCTTT9450.010.75256714
ACCGTGC803.777477E-410.6859158
GGACGTG33600.010.5167336
AGGACGT34300.010.5027085
TTAGGAC20650.010.3564213
GATATAC5150.010.3480771
AGGACCT20550.010.3145265
Produced by FastQC (version 0.11.5)