FastQCFastQC Report
Thu 26 May 2016
SRR1512679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912994
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT65870.3443293601548149No Hit
GTCCTACAGTGGACATTTCTAAATT62820.32838576597731095No Hit
GTATCAACGCAGAGTACTTTTTTTT62760.3280721215016879No Hit
CTGTAGGACGTGGAATATGGCAAGA55540.2903302362683835No Hit
CTTTAGGACGTGAAATATGGCGAGG52890.27647760526169973No Hit
GGTATCAACGCAGAGTACTTTTTTT42610.2227398517716208No Hit
TATCAACGCAGAGTACTTTTTTTTT38890.20329389428299308No Hit
GTCCTACAGTGTGCATTTCTCATTT31570.16502926825698355No Hit
CTGAAGGACCTGGAATATGGCGAGA28740.1502357038234307No Hit
ATTTAGAAATGTCCACTGTAGGACG27220.14229004377431398No Hit
GTACATGGGAAGCAGTGGTATCAAC25780.13476257635936129No Hit
GTCCTTCAGTGTGCATTTCTCATTT24930.13031927962136838No Hit
TTTCTAAATTTTCCACCTTTTTCAG22460.1174075820415537No Hit
CTGTAGGACCTGGAATATGGCGAGA21560.1127029149072083No Hit
GAATATGGCAAGAAAACTGAAAATC21450.11212790003523274No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20450.10690049210818225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9900.012.8408624
TAGAAAT9900.012.3617264
GTGTAGG4850.012.1494311
AAATGTC8550.012.0944187
GGCGAGG14650.012.03468319
TTTAGAA10400.011.9498522
ATCCCGT1201.0320946E-811.85115310
TCCAACG1701.8189894E-1211.70649918
CACCTTT8700.011.45362914
ATTTAGA9650.011.3982221
CAGGACG752.1010202E-411.3842874
CCACCTT8600.011.25191113
GTGCTAC1404.969479E-911.223761
GAAATGT10250.011.1991766
AATGTCC10100.011.143368
ACCTTTT9100.010.84588515
TTAGAAA10850.010.8421783
ATAGGAC2950.010.6124713
ATGTCCA10750.010.4731459
ACCGGGG1451.46701495E-810.47188915
Produced by FastQC (version 0.11.5)