FastQCFastQC Report
Thu 26 May 2016
SRR1512679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912994
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69670.3641935102776067No Hit
GTCCTAAAGTGTGTATTTCTCATTT69340.36246846566168006No Hit
CTGTAGGACGTGGAATATGGCAAGA66870.34955676808186537No Hit
GTCCTACAGTGGACATTTCTAAATT65530.3425520414596177No Hit
CTTTAGGACGTGAAATATGGCGAGG63650.3327245145567629No Hit
GGTATCAACGCAGAGTACTTTTTTT52240.2730797901091169No Hit
TATCAACGCAGAGTACTTTTTTTTT49250.257449840407236No Hit
CTGAAGGACCTGGAATATGGCGAGA36090.1886571520872517No Hit
GTCCTACAGTGTGCATTTCTCATTT34410.17987510676980692No Hit
ATTTAGAAATGTCCACTGTAGGACG31050.1623110161349173No Hit
TTTCTAAATTTTCCACCTTTTTCAG29070.15196074843935736No Hit
GTACATGGGAAGCAGTGGTATCAAC28160.14720380722574142No Hit
CTGTAGGACCTGGAATATGGCGAGA27160.14197639929869096No Hit
GTCCTTCAGTGTGCATTTCTCATTT25260.13204432423729504No Hit
GAATATGGCAAGAAAACTGAAAATC24290.12697373854805608No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23740.12409866418817833No Hit
GGAATATGGCGAGAAAACTGAAAAT22570.11798259691352926No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21180.1107164998949291No Hit
CCCATGTACTCTGCGTTGATACCAC20310.10616865499839519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG551.9705262E-413.80989516
TAGGACC10850.012.7038444
TGTCGAG1551.8189894E-1212.25071218
CACCTTT8150.012.11574514
AATGTCC8600.011.9290018
ACCTTTT8700.011.8951615
CCACCTT8300.011.78485813
ATGTCGA1701.8189894E-1211.72825517
AAATGTC8600.011.597647
GAAATGT9150.011.5240036
GGCGAGG17900.011.50985219
TATGTCG1701.2732926E-1111.16976716
TTCCACC8550.010.8874811
GGCCTAA700.00146549710.8802071
TGTCCAC9350.010.87025210
TCCAACG1951.8189894E-1210.7115218
ACACCGT803.7733052E-410.6869566
GACGTGA22800.010.5405797
AGGACCT24750.010.4856595
GTATAAC1002.3436281E-510.47219851
Produced by FastQC (version 0.11.5)