FastQCFastQC Report
Thu 26 May 2016
SRR1512678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414489
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59530.24655320442544984No Hit
GTCCTACAGTGGACATTTCTAAATT56470.2338797153352117No Hit
GTCCTAAAGTGTGTATTTCTCATTT51130.21176323437381572No Hit
CTGTAGGACGTGGAATATGGCAAGA50410.20878123694081852No Hit
CTTTAGGACGTGAAATATGGCGAGG42860.17751168052536168No Hit
GGTATCAACGCAGAGTACTTTTTTT37370.15477395009875797No Hit
TATCAACGCAGAGTACTTTTTTTTT34490.1428459603667691No Hit
GTCCTACAGTGTGCATTTCTCATTT30310.12553380860297975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10100.013.0552854
CATTTCG802.8959652E-511.86369714
AGGACCT20850.011.3743585
GTCCTAG3250.011.1776961
GGCGAGG13350.010.86234119
TAGACGT700.00151009610.8413884
TCGCGCG700.001542660510.8136919
ATCGCGC700.001546673410.8103238
TGTAGGA33450.010.8047012
GGACCTG20550.010.7094986
CTAGGAC3400.010.6020183
GTAGGAC32950.010.59439853
AGGACGT31850.010.483985
CGTATCA750.00201427510.47279551
GGACGTG31450.010.4665066
GTCTAAT1603.592504E-910.4318871
GTCTAGG2550.010.395791
TTAGGAC17900.010.3339293
TAGGACA8100.010.306014
GTCTTAG2103.6379788E-1210.2857821
Produced by FastQC (version 0.11.5)