FastQCFastQC Report
Thu 26 May 2016
SRR1512678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414489
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73730.30536482046511704No Hit
GTCCTACAGTGGACATTTCTAAATT60400.2501564513236548No Hit
CTGTAGGACGTGGAATATGGCAAGA59700.2472572871526853No Hit
GTCCTAAAGTGTGTATTTCTCATTT53760.22265580833045834No Hit
CTTTAGGACGTGAAATATGGCGAGG52090.21573923095114536No Hit
TATCAACGCAGAGTACTTTTTTTTT49620.2055093230907244No Hit
GGTATCAACGCAGAGTACTTTTTTT49390.20455674057740583No Hit
GTCCTACAGTGTGCATTTCTCATTT31630.13100080389680796No Hit
CTGAAGGACCTGGAATATGGCGAGA29140.1206880627743593No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25120.10403857710679154No Hit
CTGTAGGACCTGGAATATGGCGAGA24820.10279607817637604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG655.425696E-513.1613814
TAGGACC10000.012.7372924
CCGTCCC550.003073278812.0888139
CCTATAC1800.011.6177623
GCGTGCG752.0780652E-411.3980249
GGCGAGG14800.011.35645919
CTACACG951.3523373E-511.0063014
AAGGCGT1252.2443965E-710.6390376
TGTAGGA31450.010.579492
GGTATCA19250.010.4308441
AGGACCT19900.010.4132125
GGACGTG34200.010.3323986
TTATACT2400.010.2975634
GACGTGA18450.010.2963187
AGGACGT34900.010.2682695
CTGTAGG32050.010.2140541
GCGCCAC1409.739961E-810.17533113
GTAGGAC32700.010.1449513
TTAGGAC21650.010.1420653
GGACCTG20000.010.0690896
Produced by FastQC (version 0.11.5)