FastQCFastQC Report
Thu 26 May 2016
SRR1512677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313374
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74070.32018169133049823No Hit
GTCCTAAAGTGTGTATTTCTCATTT69680.3012050796801555No Hit
GTATCAACGCAGAGTACTTTTTTTT63070.2726320949401178No Hit
CTGTAGGACGTGGAATATGGCAAGA61870.26744486624298536No Hit
CTTTAGGACGTGAAATATGGCGAGG53460.23109103845724904No Hit
GGTATCAACGCAGAGTACTTTTTTT41420.17904584386268713No Hit
TATCAACGCAGAGTACTTTTTTTTT37340.16140926629243693No Hit
GTCCTACAGTGTGCATTTCTCATTT35990.155573634008163No Hit
GTACATGGGAAGCAGTGGTATCAAC34380.14861410217284363No Hit
CTGAAGGACCTGGAATATGGCGAGA30530.1319717434362105No Hit
ATTTAGAAATGTCCACTGTAGGACG26970.11658296496805098No Hit
GTCCTTCAGTGTGCATTTCTCATTT25760.11135250936510914No Hit
TTTCTAAATTTTCCACCTTTTTCAG23990.10370134703683884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA1102.7903297E-912.94151116
TCCAACG1700.012.8204918
TAGGACC11700.012.8114154
CGTGCCG550.003113658212.06771910
TAGAAAT9850.011.8466274
GGCGAGG16850.011.75720619
AAATGTC9650.011.6989137
ACCTTTT9750.011.68054315
AACCGTG658.119186E-411.6762267
CTAGGAC3850.011.5811993
GTTTAGG2900.011.5118031
GTGTAGG4300.011.4173151
AATGTCC10400.011.3691958
CGTATCA700.00113229611.2215891
GCGTCGG600.005988731511.0553518
AGGACCT23150.011.0236935
GTATAAG2500.010.9971581
GAAATGT11050.010.9893896
GTCCTAC36550.010.8531661
GCCTAGG1905.456968E-1210.8524581
Produced by FastQC (version 0.11.5)