FastQCFastQC Report
Thu 26 May 2016
SRR1512677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313374
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA76630.33124777921771403No Hit
GTCCTACAGTGGACATTTCTAAATT75670.32709799626000813No Hit
GTCCTAAAGTGTGTATTTCTCATTT73970.3197494222724038No Hit
GTATCAACGCAGAGTACTTTTTTTT68510.2961475317004514No Hit
CTTTAGGACGTGAAATATGGCGAGG66700.2883234617489433No Hit
TATCAACGCAGAGTACTTTTTTTTT47760.20645170214587008No Hit
GGTATCAACGCAGAGTACTTTTTTT46670.20173996941264144No Hit
GTACATGGGAAGCAGTGGTATCAAC39070.1688875209974695No Hit
GTCCTACAGTGTGCATTTCTCATTT38250.1653429147210957No Hit
CTGAAGGACCTGGAATATGGCGAGA36990.15989632458910666No Hit
ATTTAGAAATGTCCACTGTAGGACG32210.13923386361219586No Hit
TTTCTAAATTTTCCACCTTTTTCAG29420.127173556891363No Hit
CTGTAGGACCTGGAATATGGCGAGA28390.12272118559299103No Hit
CCCATGTACTCTGCGTTGATACCAC27870.12047338649090031No Hit
GTCCTTCAGTGTGCATTTCTCATTT27110.1171881416493831No Hit
GAATATGGCAAGAAAACTGAAAATC26890.1162371497215755No Hit
GGAATATGGCGAGAAAACTGAAAAT24280.10495492730531249No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23470.10145354793474812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG307.7317405E-418.9972769
TAGGACC13150.012.8669314
TAGAAAT9500.012.4073434
TACACCG951.0330768E-612.0071075
TCCAACG1850.011.80477818
AAATGTC10000.011.7783117
AATGTCC10350.011.4717858
GTCCTAG3450.011.3095111
GGCGAGG17950.011.2138419
GAAATGT10350.011.197436
ACCTTTT9500.011.0945715
ACTATAC1900.011.0062763
CTACACT4500.010.9842794
TGTCCAC12050.010.87810810
ATAGGAC3800.010.7561333
AGGACCT25800.010.7214625
TGTAGGA41700.010.7151662
ATGTCCA11200.010.6859679
ATTTAGA10800.010.6620491
GCACCGT1252.2431777E-710.6393956
Produced by FastQC (version 0.11.5)