FastQCFastQC Report
Thu 26 May 2016
SRR1512676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745251
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58420.33473695187683605No Hit
GTCCTACAGTGGACATTTCTAAATT51830.2969773402221228No Hit
GTCCTAAAGTGTGTATTTCTCATTT50330.28838258794866756No Hit
CTGTAGGACGTGGAATATGGCAAGA45690.2617961542494461No Hit
CTTTAGGACGTGAAATATGGCGAGG39270.2250106145190577No Hit
GGTATCAACGCAGAGTACTTTTTTT38740.22197380204910352No Hit
TATCAACGCAGAGTACTTTTTTTTT33420.19149108065258236No Hit
GTACATGGGAAGCAGTGGTATCAAC29470.1688582329991503No Hit
GTCCTACAGTGTGCATTTCTCATTT20380.11677403422201162No Hit
CCCATGTACTCTGCGTTGATACCAC20260.1160864540401352No Hit
CTGAAGGACCTGGAATATGGCGAGA19790.11339343166111925No Hit
GTCCTTCAGTGTGCATTTCTCATTT19530.11190367460038698No Hit
ATTTAGAAATGTCCACTGTAGGACG17760.10176186691770983No Hit
TTTCTAAATTTTCCACCTTTTTCAG17640.10107428673583341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA1101.6771082E-913.38951
TAGGACC7900.012.6103164
GTACTAG1001.2964356E-611.7827611
GTGTAGG3200.011.6600241
GTTCTAA2050.011.4953771
GGCGAGG12800.011.25341519
ACTGTTC5000.010.7818568
TAGAAAT7350.010.7140714
CACCTTT7600.010.61467814
GCCCTAT650.00833284210.5742721
GATATAC3900.010.5742721
GTCCTAC25750.010.5625391
AATGTCC7300.010.4942368
TCCTACA29200.010.3975472
CCACCTT7550.010.30429213
GTATAGT1052.8541408E-510.2865381
TGGCGAG27000.010.281157518
AAATGTC7200.010.2784087
GTCCTAA15000.010.2771851
GACGTGA16000.010.2586427
Produced by FastQC (version 0.11.5)