FastQCFastQC Report
Thu 26 May 2016
SRR1512676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745251
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74270.42555483423301294No Hit
CTGTAGGACGTGGAATATGGCAAGA54380.3115884190869967No Hit
GTCCTACAGTGGACATTTCTAAATT54010.3094683801928777No Hit
GTCCTAAAGTGTGTATTTCTCATTT52880.3029936668135414No Hit
TATCAACGCAGAGTACTTTTTTTTT48210.27623533806885087No Hit
GGTATCAACGCAGAGTACTTTTTTT47920.27457368596264947No Hit
CTTTAGGACGTGAAATATGGCGAGG46280.2651767568103384No Hit
GTACATGGGAAGCAGTGGTATCAAC33400.19137648395560294No Hit
CTGAAGGACCTGGAATATGGCGAGA25430.14570970020931087No Hit
CCCATGTACTCTGCGTTGATACCAC24310.13929228517846431No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23810.13642736775397923No Hit
GTCCTACAGTGTGCATTTCTCATTT22870.13104132299594728No Hit
TTTCTAAATTTTCCACCTTTTTCAG21400.12261846576796116No Hit
ATTTAGAAATGTCCACTGTAGGACG20760.11895137146462027No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19500.11173177955491789No Hit
GTCCTTCAGTGTGCATTTCTCATTT19470.11155988450944879No Hit
GAATATGGCAAGAAAACTGAAAATC19080.10932524891835044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTCG350.002171393716.2845347
TAGGACC7900.012.5143914
TAAGACT1001.4307443E-712.3579624
TCCAACG1406.85759E-1011.52926118
AGGACCT17450.011.1134955
CAAGTCG3000.011.0831666
TTTATAC2600.010.9682893
TAGCGAG700.001498540410.851068518
GGACCTG17000.010.8410126
GGCGAGG13500.010.83097519
TATGTCG1502.1518645E-910.76064416
CCAACGA1158.880743E-710.73312419
ACCTTTT7550.010.68999415
AAGTCGT3200.010.6867277
TTATACT2150.010.6114864
TGTAGGA26750.010.5547662
GTCGAGG1355.7338184E-810.54965119
GTTCTAG2001.8189894E-1210.464241
GGACGGG1002.3992337E-510.4498426
TTTACAC1553.6252459E-910.4257733
Produced by FastQC (version 0.11.5)