FastQCFastQC Report
Thu 26 May 2016
SRR1512675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114332
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65560.31007429296818095No Hit
GTCCTACAGTGGACATTTCTAAATT65460.3096013303492545No Hit
GTCCTAAAGTGTGTATTTCTCATTT56160.26561580678909463No Hit
CTGTAGGACGTGGAATATGGCAAGA55310.2615956245282198No Hit
CTTTAGGACGTGAAATATGGCGAGG48540.22957605522689908No Hit
GGTATCAACGCAGAGTACTTTTTTT40530.1916917494508904No Hit
TATCAACGCAGAGTACTTTTTTTTT38200.1806717204299041No Hit
GTCCTACAGTGTGCATTTCTCATTT35050.16577339793372092No Hit
CTGAAGGACCTGGAATATGGCGAGA25030.11838254351729056No Hit
CTGTAGGACCTGGAATATGGCGAGA23680.11199754816178349No Hit
GTCCTTCAGTGTGCATTTCTCATTT23640.1118083631142129No Hit
GAATATGGCAAGAAAACTGAAAATC21610.10220722195000596No Hit
ATTTAGAAATGTCCACTGTAGGACG21300.10074103783133397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10100.013.80896954
TCCAACG1550.012.835518
GGCGAGG15700.011.83028419
GGTATCA15450.011.6930061
GGACGTA658.112495E-411.6772866
CGACTAA1157.181188E-811.55310817
ATGTCGA1501.8007995E-1011.38806417
GGACGGG1001.9605322E-611.3853556
GTTTAGG2700.011.2728771
GTCGAGG1105.1338793E-711.19926219
TGTAGGA36750.011.126922
CGGTTTC1451.2441888E-911.12388713
AGGACCT21000.011.0239155
GTAGGAC35350.011.0039713
ATCCTAC2950.010.9831951
GGACCTG20850.010.9212046
TGTCGAG1408.723873E-910.82722518
CGTGCAA700.001545367910.8113029
CCAACGT1159.108553E-710.712337519
ACCTTTT8450.010.66954415
Produced by FastQC (version 0.11.5)