FastQCFastQC Report
Thu 26 May 2016
SRR1512675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114332
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76420.3614380333835935No Hit
GTCCTACAGTGGACATTTCTAAATT68120.3221821360126981No Hit
CTGTAGGACGTGGAATATGGCAAGA67140.3175471023472189No Hit
GTCCTAAAGTGTGTATTTCTCATTT61680.29172334335383465No Hit
CTTTAGGACGTGAAATATGGCGAGG58290.275689910572228No Hit
GGTATCAACGCAGAGTACTTTTTTT51400.24310278612819555No Hit
TATCAACGCAGAGTACTTTTTTTTT50110.23700156834404437No Hit
GTCCTACAGTGTGCATTTCTCATTT37650.17807042602580864No Hit
CTGAAGGACCTGGAATATGGCGAGA33200.15702358948358158No Hit
CTGTAGGACCTGGAATATGGCGAGA30120.1424563408206469No Hit
ATTTAGAAATGTCCACTGTAGGACG24490.11582854537508774No Hit
GAATATGGCAAGAAAACTGAAAATC24400.11540287901805393No Hit
TTTCTAAATTTTCCACCTTTTTCAG24280.11483532387534219No Hit
GTCCTTCAGTGTGCATTTCTCATTT24220.11455154630398631No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22800.10783547711523073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.013.140911
TAGGACC11250.012.674564
TCCAACG1701.2732926E-1111.17047418
GCGCGCC600.00588273111.08184211
TGTCGAG1658.185452E-1110.93352618
CCACCTT8650.010.86982713
TATGTCG1408.421921E-910.85157516
CACCTTT8600.010.82079914
GGCGAGG16150.010.81772319
ATAGGAC3500.010.592313
TGTAGGA37050.010.4431422
AGGACCT22200.010.4053745
ACCTTTT8900.010.34886815
ATGTCGA1752.382876E-1010.30899617
TTAATAC2500.010.2663923
TAGGACT4650.010.2214174
AAATGTC8950.010.1885747
TTAGGAC22050.010.1741363
GTAGGAC37500.010.13964753
CTGTAGG36700.010.1381211
Produced by FastQC (version 0.11.5)