FastQCFastQC Report
Thu 26 May 2016
SRR1512674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460175
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66260.2693304338106029No Hit
GTATCAACGCAGAGTACTTTTTTTT64600.2625829463351184No Hit
GTCCTAAAGTGTGTATTTCTCATTT59230.24075523082707528No Hit
CTGTAGGACGTGGAATATGGCAAGA57180.23242249027000111No Hit
CTTTAGGACGTGAAATATGGCGAGG46100.18738504374688794No Hit
GTCCTACAGTGTGCATTTCTCATTT42450.1725487008038046No Hit
GGTATCAACGCAGAGTACTTTTTTT39930.16230552704584025No Hit
TATCAACGCAGAGTACTTTTTTTTT37200.15120875547471216No Hit
CTGTAGGACCTGGAATATGGCGAGA28170.11450404950867316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCG653.4242657E-614.5945847
TAGGACC13400.013.4509774
GGATACG450.009262484513.0898191
ATACCGT604.1429902E-412.648646
GTGTAGG4950.012.2964961
GGTATCA15200.011.8841771
GGACCTG22450.011.5358976
GTCCTAG3800.011.3674751
AGGACCT23200.011.285645
ATCCTAC3400.011.2610931
AAATGTC8400.011.1804937
ATTACCC855.4033353E-511.1605643
GGCGAGG15600.011.05829219
AATCCCG2250.010.95297519
TGTAGGA39250.010.9245572
GTCCTAC35600.010.8928611
GAAATGT9200.010.826966
CTAGGAC3700.010.7684363
GTTCTAA3650.010.7587551
TCCTACA39650.010.6229422
Produced by FastQC (version 0.11.5)