FastQCFastQC Report
Thu 26 May 2016
SRR1512674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460175
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73270.2978243417643054No Hit
CTGTAGGACGTGGAATATGGCAAGA72160.29331246760901153No Hit
GTCCTACAGTGGACATTTCTAAATT67840.27575274116678694No Hit
GTCCTAAAGTGTGTATTTCTCATTT63370.2575833020008739No Hit
CTTTAGGACGTGAAATATGGCGAGG57140.2322599002103509No Hit
GGTATCAACGCAGAGTACTTTTTTT49140.19974188828030529No Hit
TATCAACGCAGAGTACTTTTTTTTT47240.19201886044691943No Hit
GTCCTACAGTGTGCATTTCTCATTT43580.17714186998892356No Hit
CTGTAGGACCTGGAATATGGCGAGA37060.15063969026593635No Hit
CTGAAGGACCTGGAATATGGCGAGA32440.13186053837633502No Hit
ATTTAGAAATGTCCACTGTAGGACG25010.10165943479630514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG400.005286336414.247539
TAGGACC14350.013.7791334
GGTATCA16700.012.4238021
GAACCGC550.003070968412.0900416
AACCGCT550.003072839512.0890597
TAAGACG657.9819816E-411.7000334
GGCGAGG17050.011.6948119
TGTAGGA39450.011.5443552
GTAGGAC39200.011.3735823
CACCTTT8550.011.32768314
AGGACGT37950.011.2980055
GGACGTG37400.011.2518666
CTGTAGG39900.011.1154621
AATGTCC9350.011.0729478
GACGTGG17400.011.0270477
AGGACCT27000.010.98570255
GGACCTG25300.010.963646
TTAGGAC23650.010.7724413
GACGTGA20950.010.7453737
AAGACCG803.7472864E-410.6952145
Produced by FastQC (version 0.11.5)