FastQCFastQC Report
Thu 26 May 2016
SRR1512673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553045
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62760.2458241041579761No Hit
GTCCTACAGTGGACATTTCTAAATT61210.23975292249059457No Hit
GTCCTAAAGTGTGTATTTCTCATTT58100.22757139024184844No Hit
CTGTAGGACGTGGAATATGGCAAGA50930.19948727891596113No Hit
CTTTAGGACGTGAAATATGGCGAGG45840.17955030169855996No Hit
GGTATCAACGCAGAGTACTTTTTTT39080.15307211584598002No Hit
GTCCTACAGTGTGCATTTCTCATTT37720.14774514354427754No Hit
TATCAACGCAGAGTACTTTTTTTTT35480.13897130681206166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAT250.00609910718.96424110
TTAACGT350.002191265316.2627494
TAGGACC10800.012.9123224
GTCTTAA2500.011.3934521
GTCTTAG2950.011.3202031
GGTATCA17100.011.0855831
GTACTAA1603.0559022E-1011.0496851
GGCGAGG14000.010.83265619
TCGCGCG700.001544965310.8117999
ATCGCGC700.001546231310.8107388
GTACTAG1552.1154847E-910.7724521
GTCCTAC34850.010.6815371
TGTAGGA34950.010.5314152
GATATAC4200.010.5235091
GCGCCAC1002.4376775E-510.43526413
AGGACCT21550.010.4332695
GTCCTAG3400.010.3997021
TAGACAG3750.010.3722255
TCCTACA39250.010.3202542
AAATGTC8850.010.2907557
Produced by FastQC (version 0.11.5)