FastQCFastQC Report
Thu 26 May 2016
SRR1512673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553045
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75410.29537278034660575No Hit
GTCCTACAGTGGACATTTCTAAATT63880.250211022524084No Hit
CTGTAGGACGTGGAATATGGCAAGA63710.24954515098637117No Hit
GTCCTAAAGTGTGTATTTCTCATTT60260.23603187566219944No Hit
CTTTAGGACGTGAAATATGGCGAGG57190.22400701906938578No Hit
TATCAACGCAGAGTACTTTTTTTTT51120.20023148828164014No Hit
GGTATCAACGCAGAGTACTTTTTTT49830.19517869837781943No Hit
GTCCTACAGTGTGCATTTCTCATTT39590.15506973045911843No Hit
CTGTAGGACCTGGAATATGGCGAGA31820.12463548429424473No Hit
CTGAAGGACCTGGAATATGGCGAGA30740.1204052415840692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13700.013.8762124
GGTATCA19850.011.7929291
GACCGTG1001.9346553E-611.3982017
AGGACCT24750.011.3682865
GGACCGT1354.7384674E-911.2583666
TGTAGGA39050.011.198452
TACGCTG600.00585178811.0898415
TCGCGCG600.00588431711.0815859
TGTCGAG1553.1650416E-1011.027060518
CTGTAGG38900.010.9101911
GTAGGAC39000.010.8700553
GGACCTG24650.010.7902186
AATCCCG1855.456968E-1210.77870419
AATGTCC8700.010.6994618
TATGTCG1451.4548277E-810.47802516
GGCGAGG16000.010.44507819
GACGTGG17850.010.4297277
GGACGTG36350.010.4269576
AGGACGT36750.010.4238045
CCGGTTT1303.853438E-710.22895412