FastQCFastQC Report
Thu 26 May 2016
SRR1512672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339519
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61710.2637721685525956No Hit
GTCCTACAGTGGACATTTCTAAATT46780.19995563190553273No Hit
GTCCTAAAGTGTGTATTTCTCATTT43230.18478157262240658No Hit
CTGTAGGACGTGGAATATGGCAAGA41600.17781432850085852No Hit
GGTATCAACGCAGAGTACTTTTTTT38530.16469197300812688No Hit
TATCAACGCAGAGTACTTTTTTTTT36060.1541342472533884No Hit
CTTTAGGACGTGAAATATGGCGAGG35500.15174059283126146No Hit
GTACATGGGAAGCAGTGGTATCAAC23830.10185854442729468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT400.005317258614.23496214
CGTGCCG400.00534921214.22211110
GCGCAGA1051.7673847E-712.159411
GTGTAGG3750.011.2614841
TAGGACC6750.011.2447164
ATTTAGA7600.010.9840821
AATGTCC6700.010.8682768
GTCTTAA2350.010.8658561
CCATGCG700.001546903510.8101029
GTCTTAC2100.010.7564021
GTCCAGG4550.010.5765291
CTATACT3150.010.5419224
GTCCTAG2350.010.4479391
GCACCGT1002.4342467E-510.4365026
TAGAAAT8250.010.350254
TCGAACT1401.0019539E-710.15559519
AAATGTC7300.010.1375747
GGCGAGG11800.010.12116919
CTAATAC1502.4958354E-810.1202443
GAAATGT7900.010.0882186
Produced by FastQC (version 0.11.5)