FastQCFastQC Report
Thu 26 May 2016
SRR1512672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339519
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72890.31155976933720136No Hit
CTGTAGGACGTGGAATATGGCAAGA50990.21795078390045133No Hit
GGTATCAACGCAGAGTACTTTTTTT48930.2091455551333415No Hit
GTCCTACAGTGGACATTTCTAAATT48180.20593976796085006No Hit
TATCAACGCAGAGTACTTTTTTTTT47200.2017508727221279No Hit
GTCCTAAAGTGTGTATTTCTCATTT46460.19858782937860303No Hit
CTTTAGGACGTGAAATATGGCGAGG43770.18708973938660042No Hit
GTACATGGGAAGCAGTGGTATCAAC25920.11079200468130414No Hit
CTGAAGGACCTGGAATATGGCGAGA24250.10365378524388986No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23900.10215775123006055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7450.012.759954
GCGATAA752.0778379E-411.39812711
TAAACCG600.0058479311.090765
TTAGGAC15050.010.9273493
GGCGAGG11750.010.74705919
ATAAGCG803.7876525E-410.682775514
GGACCGA909.524407E-510.554956
CGACTAA1451.4571924E-810.4767817
GGTATCA20800.010.3867381
AGGACCT16450.010.3443145
TAGAAAT8150.010.1476824
CGTCGGT856.630273E-410.05308719
ACCTTTT7000.010.03798915
CGATAAG951.6477126E-49.99814312
GTGTAAC2105.456968E-129.97057151
AGGACCG1151.0187063E-59.9196865
GACGTGA14550.09.8574877
TGTCCAC8300.09.84175710
GCGACTA1451.6303966E-79.82240116
GTCCTAA13200.09.8057691
Produced by FastQC (version 0.11.5)