FastQCFastQC Report
Thu 26 May 2016
SRR1512671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675185
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59770.3567964135304459No Hit
GGTATCAACGCAGAGTACTTTTTTT39590.2363321066031513No Hit
TATCAACGCAGAGTACTTTTTTTTT35060.2092903171888478No Hit
GTACATGGGAAGCAGTGGTATCAAC26770.1598032456116787No Hit
GTCCTAAAGTGTGTATTTCTCATTT26110.15586338225330337No Hit
GTCCTACAGTGGACATTTCTAAATT25910.1546694842659169No Hit
CTGTAGGACGTGGAATATGGCAAGA21980.1312093888137728No Hit
CTTTAGGACGTGAAATATGGCGAGG21850.1304333551219716No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18350.10954014034270843No Hit
CCCATGTACTCTGCGTTGATACCAC18100.10804776785847534No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17750.10595844638054902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAT456.82615E-414.75958217
TAGGGCC707.3585834E-613.5543114
ATCGCCG500.001529094213.26569619
TCGCATT500.001531514613.26291118
GTCCGGA450.00922866313.0967131
TAGGACC5850.012.6506894
GTGTAGG3150.011.8494071
CTAAGGT1251.8661012E-811.3856214
GTCCTAA8100.011.2777241
CTAATAC951.3794042E-510.9861263
TCTACAC2600.010.9477123
TCGAACT1303.3922333E-810.93326519
AGGACCT10750.010.7677965
CTATACT1158.9605055E-710.7255854
TCCAACG1159.138839E-710.70918318
GTAGGAC16300.010.5357743
CTACTAT951.1731603E-410.339511
TGTAGGA17350.010.335652
CCGCCCT1201.5441656E-610.28084715
GGACCTG10800.010.1908346
Produced by FastQC (version 0.11.5)