FastQCFastQC Report
Thu 26 May 2016
SRR1512671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675185
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73530.4389365950626349No Hit
GGTATCAACGCAGAGTACTTTTTTT52360.3125624930977773No Hit
TATCAACGCAGAGTACTTTTTTTTT50840.3034888683936401No Hit
GTACATGGGAAGCAGTGGTATCAAC30550.18236791757328294No Hit
GTCCTACAGTGGACATTTCTAAATT27400.16356402427194608No Hit
GTCCTAAAGTGTGTATTTCTCATTT27230.16254921098266759No Hit
CTGTAGGACGTGGAATATGGCAAGA25630.15299802708357582No Hit
CTTTAGGACGTGAAATATGGCGAGG24400.14565555446114906No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23680.14135752170655777No Hit
CCCATGTACTCTGCGTTGATACCAC21260.12691135605918152No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21050.12565776317242575No Hit
GTCCTACAGTGTGCATTTCTCATTT17380.10374973510388405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5800.012.9482654
GGTCTAT853.8626004E-612.3170211
GGCGAGG6950.011.4752519
GGTGCGT600.005875637311.0834216
GTATTAA1855.456968E-1210.8038741
TGTAGGA16950.010.6573262
TATACAG3050.010.597215
GACGTGG8150.010.37307557
GTAGGAC16900.010.2935193
GTCCTAA8800.010.2747881
TTAGGAC11000.010.1973853
GGACGTG16800.010.1221036
CTGTAGG17450.010.0903971
AAGACGA856.5533136E-410.0655165
GACCCGA856.5930345E-410.0589036
TATGTCG856.62751E-410.05319816
CGCCCTA1701.5734258E-910.05319816
TGGCGAG16250.010.04976118
ATAGGAC2650.010.0441143
TCCCGCC1803.965397E-1010.02366513
Produced by FastQC (version 0.11.5)