FastQCFastQC Report
Thu 26 May 2016
SRR1512670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480913
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65490.2639753993791802No Hit
GGTATCAACGCAGAGTACTTTTTTT39510.15925588684488332No Hit
TATCAACGCAGAGTACTTTTTTTTT36240.14607525535961963No Hit
GTCCTACAGTGGACATTTCTAAATT32170.12967000455074404No Hit
GTACATGGGAAGCAGTGGTATCAAC32070.12926692713529253No Hit
GTCCTAAAGTGTGTATTTCTCATTT30310.12217276462334631No Hit
CTGTAGGACGTGGAATATGGCAAGA29020.11697306596402213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG751.5202817E-512.63313718
TATATCG550.003101543512.074065
TAGGACC6050.011.2900314
GTACTAG1501.2350938E-911.1286561
GTGTAGG3600.010.9104481
CGTTACT700.001509139810.8422332
TGGCGAG17850.010.35067818
GTCCTAT3050.010.3023241
GGCGAGG8950.010.16523519
GTCCTAC18300.010.1413511
CGAACTG750.00267932610.1192125
GTCCTAA11400.010.0778091
TAGCACT2750.010.0042224
AGTACCG1252.617513E-69.8662335
GTATTAT2209.094947E-129.8194041
GGACCTG11800.09.8083476
TGTAGGA20300.09.7206232
AGGACGT21100.09.6666065
TTAGGAC12500.09.6387453
GTCTTAC2251.2732926E-119.6011941
Produced by FastQC (version 0.11.5)