FastQCFastQC Report
Thu 26 May 2016
SRR1512670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480913
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84970.34249487990913025No Hit
GGTATCAACGCAGAGTACTTTTTTT56950.229552588099623No Hit
TATCAACGCAGAGTACTTTTTTTTT55870.2251993520127469No Hit
GTACATGGGAAGCAGTGGTATCAAC36960.14897741275087034No Hit
CTGTAGGACGTGGAATATGGCAAGA35650.14369709860845584No Hit
GTCCTACAGTGGACATTTCTAAATT33650.13563555029942606No Hit
GTCCTAAAGTGTGTATTTCTCATTT31660.12761430973194143No Hit
CTTTAGGACGTGAAATATGGCGAGG27910.11249890665251058No Hit
CCCATGTACTCTGCGTTGATACCAC27380.1103625963506177No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27360.1102819808675274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7150.012.3628234
TACGAGT550.003057861212.0969554
AAGTCGT1501.7644197E-1011.3983427
CGCCCTA1950.011.20033816
GTAGGAC18900.010.6614133
CTAGACT2150.010.6099554
TCTACAC3350.010.4977853
TGTAGGA20150.010.4734252
GCGAACC1659.331416E-1010.3619211
GTCCTAA10950.010.3418811
CTGTAGG19750.010.2631161
TAGGACA5400.010.2088074
GATATAC3550.010.1864291
TGCGATA750.00265216310.13165610
CGTCGGT750.002657934310.12900119
ATAGGAC2450.010.0866753
GACGTGG10250.010.0083017
AGGACCG1356.402515E-79.8567795
TCGCGCG1356.4610686E-79.8502219
TAGCACT3000.09.8215754
Produced by FastQC (version 0.11.5)