FastQCFastQC Report
Thu 26 May 2016
SRR1512669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384464
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79980.2363151151851519No Hit
GTATCAACGCAGAGTACTTTTTTTT72030.21282542819187913No Hit
CTGTAGGACGTGGAATATGGCAAGA68640.2028090710966345No Hit
GTCCTAAAGTGTGTATTTCTCATTT64260.18986758316826532No Hit
CTTTAGGACGTGAAATATGGCGAGG50310.14864983051969236No Hit
GTCCTACAGTGTGCATTTCTCATTT45350.13399462957797748No Hit
GGTATCAACGCAGAGTACTTTTTTT43270.12784890015080674No Hit
TATCAACGCAGAGTACTTTTTTTTT40850.12069858033650233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15400.013.2450094
TCCAACG2200.011.62825418
GGACCGA1001.963359E-611.3845476
ACCGTGC1604.9112714E-1111.2315778
CTAGTAC1701.2732926E-1111.1611563
GTGTAGG7000.011.0813641
GTCCTAC42900.010.9633271
ACGAAAT2350.010.89572812
CCGACCC700.001545453810.8115799
TGTAGGA46900.010.7411252
TCCTACA47900.010.7149432
TATACTG4350.010.6866455
GTAGGAC45400.010.6153913
GGCGAGG17600.010.60839119
AGGACGT42700.010.5980285
AGGACCT28750.010.592585
GGACCTG27350.010.5451026
GGACGTG42200.010.5437466
CCTACAG47650.010.4326943
GACGTGG22650.010.262017
Produced by FastQC (version 0.11.5)