FastQCFastQC Report
Thu 26 May 2016
SRR1512669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384464
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93290.27564187416382624No Hit
GTCCTACAGTGGACATTTCTAAATT82750.24449957216268217No Hit
CTGTAGGACGTGGAATATGGCAAGA81850.24184036231438716No Hit
GTCCTAAAGTGTGTATTTCTCATTT65600.19382685116461573No Hit
GGTATCAACGCAGAGTACTTTTTTT60850.17979213252083637No Hit
CTTTAGGACGTGAAATATGGCGAGG60790.1796148518642834No Hit
TATCAACGCAGAGTACTTTTTTTTT60210.1779011388509377No Hit
GTCCTACAGTGTGCATTTCTCATTT47810.14126313649665057No Hit
CTGTAGGACCTGGAATATGGCGAGA38690.11431647670059424No Hit
CTGAAGGACCTGGAATATGGCGAGA35570.10509788255983812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14750.013.4046514
GACCCGT500.001500756113.2993266
TACGCAG701.085236E-412.2219775
GCGCCAC1353.7471182E-1011.95913
TGTAGGA46300.011.7042162
TATGCGA658.046062E-411.68912412
GTAGGAC45100.011.4024583
ACCGGGT752.0784463E-411.3980768
CTGTAGG45650.011.2599121
TACCGTA855.3360898E-511.1745837
GGCGAGG16700.011.14353719
AGGACCT28600.011.134635
AGGACGT40900.010.9702295
GTCCTAC42200.010.8270661
GGACCTG27850.010.8127386
GACGTGG22550.010.7409817
GGACGTG41000.010.7275076
CGGTCCA803.779037E-410.6856969
TAGAAAT10350.010.6537334
TGGCGAG41950.010.61526318
Produced by FastQC (version 0.11.5)