##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512669_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3384464 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76873856539765 33.0 31.0 34.0 30.0 34.0 2 31.897111920824095 34.0 31.0 34.0 30.0 34.0 3 31.998276536550545 34.0 31.0 34.0 30.0 34.0 4 35.43100384580838 37.0 35.0 37.0 33.0 37.0 5 35.21977364805771 37.0 35.0 37.0 33.0 37.0 6 35.080036307078466 37.0 35.0 37.0 32.0 37.0 7 35.076796503079954 37.0 35.0 37.0 32.0 37.0 8 35.07391953349186 37.0 35.0 37.0 32.0 37.0 9 36.692059953954306 39.0 37.0 39.0 33.0 39.0 10 36.495053869682174 39.0 37.0 39.0 32.0 39.0 11 36.607996125826716 39.0 37.0 39.0 32.0 39.0 12 36.5488827773024 39.0 37.0 39.0 32.0 39.0 13 36.54980197750663 39.0 37.0 39.0 32.0 39.0 14 37.82661183572938 40.0 38.0 41.0 32.0 41.0 15 37.82325443556203 40.0 38.0 41.0 32.0 41.0 16 37.793992785859146 40.0 38.0 41.0 32.0 41.0 17 37.76491048508715 40.0 38.0 41.0 32.0 41.0 18 37.69831973393719 40.0 38.0 41.0 32.0 41.0 19 37.72910954289956 40.0 38.0 41.0 32.0 41.0 20 37.68095095707917 40.0 38.0 41.0 32.0 41.0 21 37.63884532380903 40.0 38.0 41.0 32.0 41.0 22 37.54532977747732 40.0 38.0 41.0 32.0 41.0 23 37.494637555607035 40.0 38.0 41.0 32.0 41.0 24 37.454537262030264 40.0 38.0 41.0 32.0 41.0 25 37.388108131745525 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4423946624636308 1101 2 0.43286730061075573 1101 3 0.4378810759731451 1101 4 0.408682651869988 1101 5 0.4263097162975029 1101 6 0.5762774044725489 1101 7 0.4424988804170269 1101 8 0.5158387627808168 1101 9 0.6316179659925609 1101 10 0.7200146156070275 1101 11 0.7067224113405146 1101 12 0.6677077338746784 1101 13 0.6954336052266328 1101 14 0.7875763567962224 1101 15 0.7622264650171005 1101 16 0.8564294810108208 1101 17 0.8556261203078179 1101 18 0.8881144343111487 1101 19 0.8977819456765985 1101 20 0.8621700663748939 1101 21 0.9617193793435987 1101 22 0.934030849485957 1101 23 0.9499640403916203 1101 24 0.9504828307540691 1101 25 0.9441640529770368 1102 1 0.38812423911111793 1102 2 0.35582638452437365 1102 3 0.3342655181469283 1102 4 0.4060437945180837 1102 5 0.37703080949560785 1102 6 0.5265822094478239 1102 7 0.4567354645829411 1102 8 0.44989650069584286 1102 9 0.5425070567181649 1102 10 0.6750089704864308 1102 11 0.4987927514353814 1102 12 0.5147438878760937 1102 13 0.5592434939641393 1102 14 0.7086526826111381 1102 15 0.654183114275348 1102 16 0.6971613913953192 1102 17 0.7260359119114099 1102 18 0.6734287634563714 1102 19 0.6403463126662032 1102 20 0.6709069154514893 1102 21 0.582231666153568 1102 22 0.6680628847569281 1102 23 0.741160017533609 1102 24 0.7359170004482891 1102 25 0.6914510837066743 1103 1 0.41511684599759846 1103 2 0.4677529417634254 1103 3 0.4018145342513719 1103 4 0.42634656673713067 1103 5 0.4559007788431728 1103 6 0.568645432082981 1103 7 0.46789213744362 1103 8 0.42320168302224914 1103 9 0.42802894224004717 1103 10 0.6070589315753026 1103 11 0.5337254113680387 1103 12 0.5376121141641335 1103 13 0.5958879455555248 1103 14 0.6940247801589479 1103 15 0.6626745736807251 1103 16 0.66839495343077 1103 17 0.6048545622512904 1103 18 0.7054554969866444 1103 19 0.710457308632158 1103 20 0.7040553216836187 1103 21 0.6522141164426216 1103 22 0.6677159603239176 1103 23 0.6628042308936273 1103 24 0.8337154447974413 1103 25 0.7860095814196555 1104 1 0.4026885264997766 1104 2 0.38749466277097255 1104 3 0.351132167232727 1104 4 0.30473035687158045 1104 5 0.2625698097300173 1104 6 0.433183094351719 1104 7 0.2806828098712657 1104 8 0.303774548046988 1104 9 0.3775750360899437 1104 10 0.5463221151184001 1104 11 0.44561647389201653 1104 12 0.4562904185840182 1104 13 0.4016991022623131 1104 14 0.4762123110687142 1104 15 0.42045201371276164 1104 16 0.557727495474083 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-1.4536100292342482 2314 15 -1.5216871293019665 2314 16 -1.64115053298665 2314 17 -1.7584866392995195 2314 18 -1.7959421578466603 2314 19 -1.5484890818958306 2314 20 -1.6809812631718728 2314 21 -1.4777880148216411 2314 22 -1.4157556003241325 2314 23 -1.3634918008807801 2314 24 -1.4649791627714066 2314 25 -1.4860702747518815 2315 1 -0.6573478428192949 2315 2 -0.6310124903353369 2315 3 -0.6627620491931374 2315 4 -0.5527367189499302 2315 5 -0.6593661461721823 2315 6 -0.7366665980718139 2315 7 -0.8269959467526391 2315 8 -0.943394043636367 2315 9 -1.1064828422717383 2315 10 -0.9353568404002175 2315 11 -1.0336146559720305 2315 12 -1.1288798494976433 2315 13 -1.1788404706823243 2315 14 -1.4223009509466635 2315 15 -1.4801901320770625 2315 16 -1.4635428734756104 2315 17 -1.5148275841604217 2315 18 -1.2165210822226413 2315 19 -1.1762133814976536 2315 20 -1.1748860839873458 2315 21 -1.1861114876062544 2315 22 -1.200737971023095 2315 23 -1.2132889863945593 2315 24 -1.1888953592599094 2315 25 -1.3806676733079186 2316 1 -1.267978772203925 2316 2 -1.135831750631212 2316 3 -1.139777616350802 2316 4 -0.9715675673988926 2316 5 -1.166825782998309 2316 6 -4.652191493762487 2316 7 -2.3012928929264334 2316 8 -1.6693976658063647 2316 9 -1.6423327564520207 2316 10 -1.5076768931080693 2316 11 -1.5180400227242146 2316 12 -1.6342204072360715 2316 13 -1.614184138264001 2316 14 -1.7875754643857675 2316 15 -1.957564776488418 2316 16 -1.8540153403986395 2316 17 -1.924757004673026 2316 18 -2.2265176833234435 2316 19 -2.0476866498033672 2316 20 -2.1502104246906057 2316 21 -2.293091092217594 2316 22 -2.3085705398532994 2316 23 -2.3944062075502686 2316 24 -2.318258608886623 2316 25 -2.345307908300434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29548.0 3 1.0 4 612.0 5 5167.0 6 6481.0 7 5281.0 8 4029.0 9 3877.0 10 3424.0 11 3289.0 12 3157.0 13 2977.0 14 3060.0 15 3023.0 16 3214.0 17 3328.0 18 3549.0 19 4240.0 20 5259.0 21 6898.0 22 8886.0 23 11018.0 24 13606.0 25 16276.0 26 18562.0 27 21269.0 28 24623.0 29 30169.0 30 38384.0 31 50472.0 32 65112.0 33 86087.0 34 116480.0 35 159301.0 36 239126.0 37 431937.0 38 1952742.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79594533889753 18.909618841259913 12.937889253798657 25.356546566043903 2 15.10281530479927 20.21086712144334 40.83772959976737 23.84858797399002 3 19.134019448869896 24.27864500848583 29.768997395156223 26.818338147488053 4 12.605799832699974 16.63205134001056 37.92542650130498 32.83672232598448 5 13.057357526025424 37.983940354111965 35.80261702012499 13.156085099737624 6 32.52498900412878 37.01981739759652 17.48677539573313 12.968418202541585 7 28.55267416069051 31.313119073408824 21.795787603976255 18.338419161924406 8 25.23979167516363 37.62744705136185 20.04739384116502 17.0853674323095 9 26.458335303630708 14.841659021912859 19.925824214797093 38.774181459659346 10 15.073375336405833 28.969854845278437 34.42186074911587 21.534909069199855 11 34.838463882512094 21.830274688394173 24.706940820089844 18.624320609003885 12 23.682460490809515 24.315050880921206 31.805289960660758 20.197198667608525 13 29.42916949017802 21.615059331418124 25.70506021504296 23.250710963360895 14 20.604717079259412 22.25571845982185 27.404016632940596 29.735547827978138 15 22.81631986129164 31.58534520635617 22.93835154402581 22.659983388326385 16 21.89901196112376 27.99924122018177 28.190265812271136 21.91148100642333 17 21.58122669654473 28.846719219199258 27.859401735539556 21.712652348716457 18 21.461035565008093 27.153487448565382 30.336686567816866 21.048790418609652 19 23.683802309814926 26.584204652663505 27.594981366121946 22.13701167139962 20 23.781815968496044 27.0618331292636 28.225562452429692 20.930788449810663 21 22.71059760127453 26.544084971800558 27.349825555833952 23.39549187109096 22 23.192665267126518 28.107119483401267 27.361650137614884 21.338565111857335 23 21.950270412094795 27.822603520084716 28.113491530712103 22.113634537108386 24 23.145805744014854 26.5926164926656 28.77006744351096 21.491510319808587 25 22.484889778706467 27.30166431080372 28.309150281994434 21.904295628495383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 421.0 3 671.0 4 671.0 5 671.0 6 2048.5 7 3426.0 8 3426.0 9 3426.0 10 4954.5 11 6483.0 12 6483.0 13 6483.0 14 10024.5 15 13566.0 16 13566.0 17 13566.0 18 24826.5 19 36087.0 20 36087.0 21 36087.0 22 58073.0 23 80059.0 24 80059.0 25 80059.0 26 117096.5 27 154134.0 28 154134.0 29 154134.0 30 203807.5 31 253481.0 32 253481.0 33 253481.0 34 288716.0 35 323951.0 36 323951.0 37 323951.0 38 348732.0 39 373513.0 40 373513.0 41 373513.0 42 394754.5 43 415996.0 44 415996.0 45 415996.0 46 433167.5 47 450339.0 48 450339.0 49 450339.0 50 434098.0 51 417857.0 52 417857.0 53 417857.0 54 371412.0 55 324967.0 56 324967.0 57 324967.0 58 278903.5 59 232840.0 60 232840.0 61 232840.0 62 192184.0 63 151528.0 64 151528.0 65 151528.0 66 117500.0 67 83472.0 68 83472.0 69 83472.0 70 61276.5 71 39081.0 72 39081.0 73 39081.0 74 27078.5 75 15076.0 76 15076.0 77 15076.0 78 10250.5 79 5425.0 80 5425.0 81 5425.0 82 3579.5 83 1734.0 84 1734.0 85 1734.0 86 1105.5 87 477.0 88 477.0 89 477.0 90 294.0 91 111.0 92 111.0 93 111.0 94 64.0 95 17.0 96 17.0 97 17.0 98 9.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15488420027513958 2 0.01571888488103286 3 0.0 4 0.0036933470115208793 5 0.10172955008533109 6 0.04065636390282183 7 0.02597161618501482 8 0.02665119203513466 9 0.026001162961106987 10 0.02665119203513466 11 0.03259009402966023 12 0.03173323752298739 13 0.019944073862212745 14 0.009927716766968122 15 0.002866037280940202 16 0.002452382415649864 17 0.0012114178197788483 18 0.0020682743264516922 19 0.00109323071541018 20 0.0 21 0.0 22 5.613887457511736E-4 23 0.0 24 5.909355218433406E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3384464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.335587155379244 #Duplication Level Percentage of deduplicated Percentage of total 1 78.89097150420153 49.966060014768956 2 13.516020578910659 17.12090198738991 3 3.738891592372614 7.104146829396912 4 1.4922310250065098 3.780453125610429 5 0.7386017957209707 2.338988920300258 6 0.40281061220567227 1.5307347981878414 7 0.25852246297402365 1.1461570389720204 8 0.16866250694246093 0.8545871126639192 9 0.13058269952385415 0.7443478752009989 >10 0.6058938133371219 6.60025396041401 >50 0.031065751789829253 1.3402552259193266 >100 0.020706798359267333 2.536356734675781 >500 0.002706053730337677 1.1582088822033263 >1k 0.0020062122356194127 2.2852721437311545 >5k 3.265926895194393E-4 1.4932753505651981 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9329 0.27564187416382624 No Hit GTCCTACAGTGGACATTTCTAAATT 8275 0.24449957216268217 No Hit CTGTAGGACGTGGAATATGGCAAGA 8185 0.24184036231438716 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6560 0.19382685116461573 No Hit GGTATCAACGCAGAGTACTTTTTTT 6085 0.17979213252083637 No Hit CTTTAGGACGTGAAATATGGCGAGG 6079 0.1796148518642834 No Hit TATCAACGCAGAGTACTTTTTTTTT 6021 0.1779011388509377 No Hit GTCCTACAGTGTGCATTTCTCATTT 4781 0.14126313649665057 No Hit CTGTAGGACCTGGAATATGGCGAGA 3869 0.11431647670059424 No Hit CTGAAGGACCTGGAATATGGCGAGA 3557 0.10509788255983812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.954677609216703E-5 2 0.0 0.0 0.0 0.0 2.954677609216703E-5 3 0.0 0.0 0.0 0.0 2.954677609216703E-5 4 0.0 0.0 0.0 2.954677609216703E-5 2.954677609216703E-5 5 0.0 0.0 0.0 2.954677609216703E-5 5.909355218433406E-5 6 0.0 0.0 0.0 2.954677609216703E-5 5.909355218433406E-5 7 0.0 0.0 0.0 2.954677609216703E-5 5.909355218433406E-5 8 0.0 0.0 0.0 2.954677609216703E-5 5.909355218433406E-5 9 0.0 0.0 0.0 2.954677609216703E-5 5.909355218433406E-5 10 0.0 0.0 0.0 5.909355218433406E-5 5.909355218433406E-5 11 0.0 0.0 0.0 5.909355218433406E-5 5.909355218433406E-5 12 0.0 0.0 0.0 5.909355218433406E-5 5.909355218433406E-5 13 0.0 0.0 0.0 5.909355218433406E-5 8.86403282765011E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1475 0.0 13.404651 4 GACCCGT 50 0.0015007561 13.299326 6 TACGCAG 70 1.085236E-4 12.221977 5 GCGCCAC 135 3.7471182E-10 11.959 13 TGTAGGA 4630 0.0 11.704216 2 TATGCGA 65 8.046062E-4 11.689124 12 GTAGGAC 4510 0.0 11.402458 3 ACCGGGT 75 2.0784463E-4 11.398076 8 CTGTAGG 4565 0.0 11.259912 1 TACCGTA 85 5.3360898E-5 11.174583 7 GGCGAGG 1670 0.0 11.143537 19 AGGACCT 2860 0.0 11.13463 5 AGGACGT 4090 0.0 10.970229 5 GTCCTAC 4220 0.0 10.827066 1 GGACCTG 2785 0.0 10.812738 6 GACGTGG 2255 0.0 10.740981 7 GGACGTG 4100 0.0 10.727507 6 CGGTCCA 80 3.779037E-4 10.685696 9 TAGAAAT 1035 0.0 10.653733 4 TGGCGAG 4195 0.0 10.615263 18 >>END_MODULE