FastQCFastQC Report
Thu 26 May 2016
SRR1512668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77420.3416149890526119No Hit
GTCCTACAGTGGACATTTCTAAATT62020.2736626404164685No Hit
GTCCTAAAGTGTGTATTTCTCATTT58330.2573805516848211No Hit
CTGTAGGACGTGGAATATGGCAAGA54800.2418044613805623No Hit
GGTATCAACGCAGAGTACTTTTTTT48750.21510889584493448No Hit
CTTTAGGACGTGAAATATGGCGAGG48510.21404989820385176No Hit
TATCAACGCAGAGTACTTTTTTTTT45310.1999299296560817No Hit
GTCCTACAGTGTGCATTTCTCATTT31800.14031718744346497No Hit
CTGAAGGACCTGGAATATGGCGAGA27100.11957848363892769No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23430.10338464471070391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATT400.005366496314.21516919
TAGGACC9750.013.330574
TACACCG905.498532E-712.6494465
ATACTCG550.003100095512.07473856
GTTCTAA2850.011.3721861
GGCGAGG15050.011.20841619
CATGCGG600.005956496611.06335510
CCGTCCA600.006062512811.0371589
CCACCTT10400.010.94687913
ACCTTTT10350.010.91100315
TAGAAAT9150.010.78313354
GGTATCA17550.010.7448151
AAATGTC8100.010.65856557
CACCTTT10700.010.64279114
AGGACCT20500.010.5977675
CCAACGA1355.898619E-810.52975519
TCCAACG1803.8198777E-1110.52672118
GGACCTG20250.010.4945886
TCAATAC2200.010.3495473
TGTAGGA35450.010.3033212
Produced by FastQC (version 0.11.5)