FastQCFastQC Report
Thu 26 May 2016
SRR1512668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266294
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99590.43943989614763135No Hit
GGTATCAACGCAGAGTACTTTTTTT68540.30243207633255No Hit
TATCAACGCAGAGTACTTTTTTTTT67780.29907858380245456No Hit
CTGTAGGACGTGGAATATGGCAAGA65120.28734135994712073No Hit
GTCCTACAGTGGACATTTCTAAATT64100.282840619972519No Hit
GTCCTAAAGTGTGTATTTCTCATTT62090.27397151472845094No Hit
CTTTAGGACGTGAAATATGGCGAGG56220.24807019742363523No Hit
GTCCTACAGTGTGCATTTCTCATTT34310.15139253777312212No Hit
CTGAAGGACCTGGAATATGGCGAGA33440.14755367132419714No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30600.1350221992380512No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26480.11684273973279725No Hit
TTTCTAAATTTTCCACCTTTTTCAG26390.11644561561739121No Hit
ATTTAGAAATGTCCACTGTAGGACG26160.11543074287802023No Hit
CTGTAGGACCTGGAATATGGCGAGA25810.11388637131810789No Hit
GAATATGGCAAGAAAACTGAAAATC24140.10651751273224039No Hit
GTCCTTCAGTGTGCATTTCTCATTT23020.10157552374052087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG1006.548362E-1014.2588315
TAGGACC13050.013.0381514
CCAACGA1353.7471182E-1011.95611419
GGTATCA21750.011.8548541
TCCAACG2300.011.55859618
CTTACAC2000.011.4065613
TTATACG600.005851951411.0897114
GATATAC7500.011.0368251
TAGAAAT10200.010.996524
TTACACC1752.0008883E-1110.8633914
TGCGATA700.001492393710.85667710
GGCGAGG15900.010.80827419
AGGACCT24750.010.7541345
TTTAGAA11700.010.7260122
GGACCTG23700.010.622616
GTACTAG1355.5648343E-810.5716711
GTGTAGG4000.010.4659551
AAGTCGT3450.010.463557
AATCCCG3750.010.3807219
CAAGTCG3300.010.3638786
Produced by FastQC (version 0.11.5)