FastQCFastQC Report
Thu 26 May 2016
SRR1512667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191962
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68290.3115473717153856No Hit
GTCCTACAGTGGACATTTCTAAATT66930.30534288459380227No Hit
GTCCTAAAGTGTGTATTTCTCATTT63930.2916565159432508No Hit
CTGTAGGACGTGGAATATGGCAAGA58220.265606794278368No Hit
CTTTAGGACGTGAAATATGGCGAGG54240.24744954520196974No Hit
GGTATCAACGCAGAGTACTTTTTTT45180.20611671187730443No Hit
TATCAACGCAGAGTACTTTTTTTTT39600.18066006618727878No Hit
GTCCTACAGTGTGCATTTCTCATTT37690.17194641147976106No Hit
CTGAAGGACCTGGAATATGGCGAGA28240.1288343502305241No Hit
GTACATGGGAAGCAGTGGTATCAAC28120.128286895484502No Hit
CTGTAGGACCTGGAATATGGCGAGA25660.11706407319104985No Hit
ATTTAGAAATGTCCACTGTAGGACG25550.11656223967386296No Hit
GTCCTTCAGTGTGCATTTCTCATTT25540.11651661844502778No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22270.10159847661592672No Hit
GAATATGGCAAGAAAACTGAAAATC22110.100868536954564No Hit
TTTCTAAATTTTCCACCTTTTTCAG22100.10082291572572882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10550.013.6681174
ATCGCGC802.1000342E-613.0060948
TCGCGCG854.129277E-612.244119
AAATGTC11450.011.765237
AATGTCC11550.011.6292198
GGCGAGG15800.011.57868619
AATCCCG3400.011.43046819
GATATAC5950.011.3932241
GAAATGT11500.011.3016096
TCCAACG1803.6379788E-1211.05570418
AGGACCT22400.010.9267055
GTATTGA1353.4731784E-810.9162131
CCACCTT8200.010.76010613
TAGAAAT12900.010.7369444
ATGTCCA12250.010.7357459
TTTAGAA13300.010.6993452
CGTGCGC803.8428555E-410.6659910
GGACCTG21900.010.6130336
ATCCTAC2800.010.5263471
TGTCCAC12750.010.48473710
Produced by FastQC (version 0.11.5)