FastQCFastQC Report
Thu 26 May 2016
SRR1512667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191962
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85650.39074582497324317No Hit
GTCCTACAGTGGACATTTCTAAATT69840.31861866218483714No Hit
GTCCTAAAGTGTGTATTTCTCATTT67170.30643779408584637No Hit
CTGTAGGACGTGGAATATGGCAAGA66500.3033811717538899No Hit
CTTTAGGACGTGAAATATGGCGAGG62940.28714001428856883No Hit
GGTATCAACGCAGAGTACTTTTTTT61780.281847951743689No Hit
TATCAACGCAGAGTACTTTTTTTTT58710.26784223449129135No Hit
GTCCTACAGTGTGCATTTCTCATTT39240.17901770194921263No Hit
CTGAAGGACCTGGAATATGGCGAGA33890.15461034452239591No Hit
GTACATGGGAAGCAGTGGTATCAAC30330.1383691870570749No Hit
ATTTAGAAATGTCCACTGTAGGACG29890.13636185298832737No Hit
CTGTAGGACCTGGAATATGGCGAGA29800.1359512619288108No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29200.13321398819870053No Hit
TTTCTAAATTTTCCACCTTTTTCAG28380.12947304743421648No Hit
GTCCTTCAGTGTGCATTTCTCATTT24970.11391620840142302No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24290.11081396484063136No Hit
GAATATGGCAAGAAAACTGAAAATC24010.10953657043324656No Hit
GGAATATGGCGAGAAAACTGAAAAT22460.10246527996379498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG400.00525636914.25964455
GCGTAGG551.9322E-413.8420781
TAGGACC11600.013.1114184
CCAACGA1650.012.65875919
GACCGGG550.00307037812.0902567
TAGAAAT9850.012.063174
GTCGAGG1501.4551915E-1112.02582219
GGCGAGG16200.011.48665119
CCACCTT9700.011.45526713
CACCTTT9900.011.41362614
AGGACCG1001.9149265E-611.4077165
TCCAACG2750.011.39288318
ACCTTTT9850.011.37386515
TATGTCG2300.011.14546716
CCGAACT600.00585042111.09007554
ATAGGAC3600.011.0900753
GACGTTA600.00587932511.0827347
AGGACGT38300.011.0205095
TTAGGAC22800.011.006693
GGACGTG37300.011.0023376
Produced by FastQC (version 0.11.5)