FastQCFastQC Report
Thu 26 May 2016
SRR1512666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038183
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT98900.32552351191485174No Hit
GTCCTAAAGTGTGTATTTCTCATTT86750.28553250413158127No Hit
CTGTAGGACGTGGAATATGGCAAGA86600.28503878798610877No Hit
GTATCAACGCAGAGTACTTTTTTTT71690.2359634031261448No Hit
CTTTAGGACGTGAAATATGGCGAGG70490.232013673962365No Hit
GTCCTACAGTGTGCATTTCTCATTT52670.173360195880235No Hit
GGTATCAACGCAGAGTACTTTTTTT43230.14228899312516724No Hit
TATCAACGCAGAGTACTTTTTTTTT41120.1353440526788544No Hit
CTGAAGGACCTGGAATATGGCGAGA38880.12797122490646545No Hit
GTCCTTCAGTGTGCATTTCTCATTT33280.10953915547549309No Hit
CTGTAGGACCTGGAATATGGCGAGA32300.10631354332507291No Hit
GAATATGGCAAGAAAACTGAAAATC30970.10193592683521698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC602.6821355E-514.1855048
TAGGACC15750.014.0950634
GTGTAGG5450.012.4367161
AGGACCT32350.011.5252425
GGCGAGG20600.011.31703319
TGGCGAA1354.940375E-911.22868318
TGTAGGA55150.011.0952922
GGACCTG32700.011.0537346
TAAGACG951.381975E-510.9850214
TCCAACG2000.010.89533218
ACCTTTT12700.010.76054715
GTAGGAC54700.010.7356393
GACCCGT803.8207008E-410.6729456
ATTTAGA11250.010.6527181
CTGTAGG52050.010.6252891
GTCCTAC48050.010.5693991
CTAGGAC4500.010.5410053
TGGCGAG48550.010.49870318
GGCGAAA1002.4658852E-510.42455119
TAGAAAT11950.010.40004354
Produced by FastQC (version 0.11.5)