FastQCFastQC Report
Thu 26 May 2016
SRR1512666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3038183
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA104080.3425731761385012No Hit
GTCCTACAGTGGACATTTCTAAATT102680.33796515878075806No Hit
GTATCAACGCAGAGTACTTTTTTTT91660.3016934792933803No Hit
GTCCTAAAGTGTGTATTTCTCATTT89140.2933990480494427No Hit
CTTTAGGACGTGAAATATGGCGAGG83680.27542778035424464No Hit
TATCAACGCAGAGTACTTTTTTTTT61720.20314773665707433No Hit
GGTATCAACGCAGAGTACTTTTTTT59670.1964002826689505No Hit
GTCCTACAGTGTGCATTTCTCATTT53760.176947866537335No Hit
CTGAAGGACCTGGAATATGGCGAGA46600.15338114919344886No Hit
CTGTAGGACCTGGAATATGGCGAGA39280.12928780129439207No Hit
GTCCTTCAGTGTGCATTTCTCATTT34080.11217230825134629No Hit
TTTCTAAATTTTCCACCTTTTTCAG33590.1105595021761362No Hit
ATTTAGAAATGTCCACTGTAGGACG33450.11009870044036188No Hit
GAATATGGCAAGAAAACTGAAAATC32620.1073668044354142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC604.099294E-412.6647367
TAGGACC15600.012.3702734
TAGAAAT13000.012.1387124
AAATGTC12300.011.969727
AATGTCC12800.011.2051388
GACCGTA600.005884521611.0816447
GAAATGT13750.011.0535886
CCACCTT11850.010.89949613
GGCGAGG24600.010.88482119
CACCTTT12300.010.80815714
TAAGACG1158.751085E-710.7461614
TGTAGGA53100.010.6175742
TTTAGAA14600.010.6145082
ACCTTTT12300.010.57620515
ATGTCCA13800.010.5306569
ATTTAGA13450.010.47091
AGGACGT54950.010.3798585
TAGGACA13800.010.3328484
TGTCCAC14450.010.25398510
CTGTAGG53600.010.2259171
Produced by FastQC (version 0.11.5)