FastQCFastQC Report
Thu 26 May 2016
SRR1512665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256018
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71390.3164425106537271No Hit
GTACATGGGAAGCAGTGGTATCAAC49740.22047696427954033No Hit
GTCCTACAGTGGACATTTCTAAATT46060.20416503769030211No Hit
GGTATCAACGCAGAGTACTTTTTTT44430.1969399180325689No Hit
TATCAACGCAGAGTACTTTTTTTTT41580.18430704010340343No Hit
GTCCTAAAGTGTGTATTTCTCATTT40750.1806279914433307No Hit
CTGTAGGACGTGGAATATGGCAAGA38730.17167416217423798No Hit
CTTTAGGACGTGAAATATGGCGAGG34320.15212644579963458No Hit
CCCATGTACTCTGCGTTGATACCAC32710.14498997791684287No Hit
GTCCTACAGTGTGCATTTCTCATTT23780.10540696040545776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC1551.8189894E-1212.24089053
TAGGACC9050.011.7406964
GTGTAGG4200.011.4637131
CGTTTCC1001.9534964E-611.38885214
ATAGGAC3250.011.38402753
TGTAGGA25550.010.6191522
GGCGAGG11500.010.466104519
GTCCTAC23350.010.1837331
GTAGCAC2151.8189894E-1210.1485513
ATTTCTA16500.010.06590515
GTTCTAT2052.1827873E-1110.06571
GTCCTAT2350.010.0351051
CTGTAGG23650.010.0130051
GTAGGAC24750.010.0041463
TTAGGAC15850.09.9355863
TAGGACA8150.09.8943144
AGGACCT17350.09.8423195
CATTTCT32550.09.82601914
GTCCTAA14000.09.7558531
GACGTGA13750.09.7283877
Produced by FastQC (version 0.11.5)