FastQCFastQC Report
Thu 26 May 2016
SRR1512665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256018
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92740.41107828040379113No Hit
GGTATCAACGCAGAGTACTTTTTTT63460.28129208188941757No Hit
TATCAACGCAGAGTACTTTTTTTTT60480.26808296742313226No Hit
GTACATGGGAAGCAGTGGTATCAAC55040.24396968463904098No Hit
GTCCTACAGTGGACATTTCTAAATT46910.20793273812531637No Hit
CTGTAGGACGTGGAATATGGCAAGA44950.1992448641810482No Hit
GTCCTAAAGTGTGTATTTCTCATTT43700.1937041282472037No Hit
CTTTAGGACGTGAAATATGGCGAGG40110.1777911346452023No Hit
CCCATGTACTCTGCGTTGATACCAC38090.16883730537610958No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30410.13479502379856897No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26190.11608949928590996No Hit
GTCCTACAGTGTGCATTTCTCATTT25650.11369590136248912No Hit
GAGTACATGGGAAGCAGTGGTATCA24710.10952926794023805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCG350.002172859916.2831869
ATAGGAC2500.011.406073
TAGGACC9200.011.3647434
GACCCGT600.00587996111.0825976
TAGGACA6100.011.0632654
AATGTCC8200.011.004398
TTTAGAA8900.011.00172
ATTTAGA8850.010.75258351
ATTATAC2150.010.6102973
AAATGTC8250.010.4773947
TCAATAC1553.6325218E-910.4249023
TGTCCAC9350.010.36179810
ATGTCCA9250.010.2686779
TAGAAAT8150.010.14650254
GGACCGC750.002649824410.132666
CGAACCG1803.947207E-1010.02599912
GAAATGT8450.010.0052526
GAACCGG1901.0004442E-109.996675513
CACCTTT7300.09.88624614
AACACCG1252.5527643E-69.885485
Produced by FastQC (version 0.11.5)