FastQCFastQC Report
Thu 26 May 2016
SRR1512664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026267
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59630.2942850078494098No Hit
GGTATCAACGCAGAGTACTTTTTTT37190.18353948418446334No Hit
TATCAACGCAGAGTACTTTTTTTTT33240.16404550831652492No Hit
GTACATGGGAAGCAGTGGTATCAAC31250.15422449262609517No Hit
GTCCTACAGTGGACATTTCTAAATT29770.14692042065532332No Hit
GTCCTAAAGTGTGTATTTCTCATTT29610.1461307912530777No Hit
CTGTAGGACGTGGAATATGGCAAGA29180.14400866223454264No Hit
CTTTAGGACGTGAAATATGGCGAGG24160.11923403973908671No Hit
CCCATGTACTCTGCGTTGATACCAC20940.10334274801889384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT2350.011.7034491
GTTTAGG1950.011.5855581
GTATTAG1801.8189894E-1211.4596281
GTTATAC1252.2826316E-710.6257233
TCCAACG1451.5059413E-810.45441818
TAGGACC6000.010.2778574
TTAGACT1856.184564E-1110.2564894
GTCTTAG2300.010.2496061
GTCCAAT1251.6764043E-610.215441
AGGCGTG1409.882933E-810.1649137
GTCCTAC16950.010.1412641
GGACGTG19750.010.1357486
AAGGCGT1502.4967449E-810.1197366
GTTCTAT2251.8189894E-1210.0408171
AGGACGT20550.09.9720025
GCGTGCG951.7188629E-49.9561819
GTATTGG1709.646101E-99.8225381
GACTTAC1001.9696106E-49.8225381
TAGACAG2650.09.6662575
GACGTGA10150.09.6274587
Produced by FastQC (version 0.11.5)