FastQCFastQC Report
Thu 26 May 2016
SRR1512664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026267
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74330.36683220918072496No Hit
GGTATCAACGCAGAGTACTTTTTTT49380.24369937426805058No Hit
TATCAACGCAGAGTACTTTTTTTTT47680.23530956186919097No Hit
GTACATGGGAAGCAGTGGTATCAAC35470.17505096811032309No Hit
CTGTAGGACGTGGAATATGGCAAGA34560.1705599508850512No Hit
GTCCTACAGTGGACATTTCTAAATT31530.155606344080025No Hit
GTCCTAAAGTGTGTATTTCTCATTT30840.1522010672828408No Hit
CTTTAGGACGTGAAATATGGCGAGG28050.13843190458118304No Hit
CCCATGTACTCTGCGTTGATACCAC25780.12722903743682346No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24150.11918468790144635No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21200.10462589579754297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5250.012.856144
CGACTAA1201.0111762E-811.86762717
TGTAGGA17600.011.1820882
ACCGCCT855.328706E-511.1755998
GTAATAC1201.2650526E-711.0901373
GTAGGAC18200.010.8115773
TGGCGAG17150.010.73968418
GGCGAGG8850.010.62052219
CTAGACA1355.6341378E-810.5625564
CGAACCG909.53688E-510.5534312
GTGTCGA909.5536016E-510.55160613
AGGACGT19950.010.5310495
GGACGTG19800.010.5077946
CTGTAGG18450.010.3169141
GTCTTAG2600.010.2494571
GCGACTA1409.779251E-810.17225316
AGGACCT12450.010.1555775
TATGGCG18500.010.05861716
ATGGCGA18650.010.02862317
TAGGACG21250.010.020764
Produced by FastQC (version 0.11.5)