FastQCFastQC Report
Thu 26 May 2016
SRR1512663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797572
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT103160.3687483289080674No Hit
CTGTAGGACGTGGAATATGGCAAGA86580.3094826513848437No Hit
GTCCTAAAGTGTGTATTTCTCATTT84360.30154719878523234No Hit
GTATCAACGCAGAGTACTTTTTTTT71410.25525705862083264No Hit
CTTTAGGACGTGAAATATGGCGAGG67850.24253173823587026No Hit
GTCCTACAGTGTGCATTTCTCATTT65480.2340601064065554No Hit
CTGTAGGACCTGGAATATGGCGAGA45180.16149718398668558No Hit
GGTATCAACGCAGAGTACTTTTTTT44160.157851165224702No Hit
TATCAACGCAGAGTACTTTTTTTTT38880.13897765633913978No Hit
CTGAAGGACCTGGAATATGGCGAGA37610.13443800552765042No Hit
GTCCTTCAGTGTGCATTTCTCATTT32430.11592194946189052No Hit
GAATATGGCAAGAAAACTGAAAATC28700.1025889592832642No Hit
ATTTAGAAATGTCCACTGTAGGACG28570.10212427061752119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC17300.013.490014
TCCAACG2850.011.96707618
GTGTAGG6200.011.8847471
GTCCTAT4800.011.4621761
TGTAGGA56200.011.41126252
AGGACCT31950.011.3723765
GTAGGAC54850.011.225133
GACGTGG26600.011.163137
AGGACGT51050.011.0757675
TAATACG600.00593841411.0680244
ACCTTTT12700.011.06034615
GGACGTG50150.010.9529466
GGCGAGG21300.010.94388919
ATCCTAC4850.010.9388681
CCGTTTC2000.010.91030113
GGACCTG32000.010.8506176
CTGTAGG50300.010.8013361
GTCCTAC52700.010.7941461
CTAGGAC4750.010.7850823
CGTTTCC2300.010.72892614
Produced by FastQC (version 0.11.5)