FastQCFastQC Report
Thu 26 May 2016
SRR1512663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797572
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT106930.3822243002146147No Hit
CTGTAGGACGTGGAATATGGCAAGA102130.3650665648641036No Hit
GTATCAACGCAGAGTACTTTTTTTT92900.33207366959635No Hit
GTCCTAAAGTGTGTATTTCTCATTT85530.3057293967769194No Hit
CTTTAGGACGTGAAATATGGCGAGG79800.28524735020224684No Hit
GTCCTACAGTGTGCATTTCTCATTT69120.24707138904735965No Hit
GGTATCAACGCAGAGTACTTTTTTT61560.22004795587030468No Hit
TATCAACGCAGAGTACTTTTTTTTT58870.21043247501762244No Hit
CTGTAGGACCTGGAATATGGCGAGA55980.20010208852533554No Hit
CTGAAGGACCTGGAATATGGCGAGA45610.16303423111183557No Hit
ATTTAGAAATGTCCACTGTAGGACG32810.11728027017713932No Hit
GTCCTTCAGTGTGCATTTCTCATTT32260.11531427966822659No Hit
GAATATGGCAAGAAAACTGAAAATC31920.11409894008089871No Hit
TTTCTAAATTTTCCACCTTTTTCAG31860.11388446838901733No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29420.10516261958584086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC18950.014.144954
GGACCGG802.0027874E-613.06148056
CCAACGA1750.013.02219919
AGGACCG1457.2759576E-1212.4550985
ACGTTAA550.003072941712.0891018
TCCAACG2300.011.97240118
GGCGAGG22350.011.72580519
GACCGGG658.0358196E-411.6907677
AGGACCT36100.011.5326325
TGTAGGA54950.011.4182612
TAGTACG752.0644133E-411.4062484
CGTGCAA752.0778307E-411.39829410
AATGCCG2750.011.394423518
AAATGTC12050.011.3512027
AGGACGT51350.011.23594955
GTAGGAC55700.011.1434473
ACCTTTT10350.011.00949915
GGACGTG51000.011.0079636
TGTACCG951.3529205E-511.0060295
GGACCGT951.3623403E-510.9991416
Produced by FastQC (version 0.11.5)