FastQCFastQC Report
Thu 26 May 2016
SRR1512662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518934
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73500.2917900985099252No Hit
GGTATCAACGCAGAGTACTTTTTTT46580.18491949372234445No Hit
TATCAACGCAGAGTACTTTTTTTTT42100.16713419247983474No Hit
GTCCTACAGTGGACATTTCTAAATT34400.13656570596927112No Hit
GTCCTAAAGTGTGTATTTCTCATTT31430.12477500402948231No Hit
CTGTAGGACGTGGAATATGGCAAGA30030.11921709739119801No Hit
CTTTAGGACGTGAAATATGGCGAGG26250.10421074946783045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG602.603553E-514.2301145
TAGGACC7450.012.2245274
GTCCTAA11350.011.1696641
ACCCGCG600.00593876211.0678667
TGTAGGA24300.010.5406152
TTGCCGC909.700243E-510.53598711
GGTATCA17950.010.5119451
GACGTGG11700.010.4597427
GGCGAGG8450.010.43165819
GTAGGAC22950.010.4166083
GGACGTG20500.010.3197256
GTGTAGG4050.010.1915581
TAGGACA7100.010.1548234
AGGACGT21150.010.1371355
TACTCCG750.002679394310.1191925
TGGCGAG20550.010.05095218
TCCCCCG951.6623686E-49.98943517
AAAACCG1054.170428E-59.9384935
AGGACCT15150.09.89376455
GTATAAT2500.09.8275731
Produced by FastQC (version 0.11.5)