FastQCFastQC Report
Thu 26 May 2016
SRR1512662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518934
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT95210.3779773507364623No Hit
GGTATCAACGCAGAGTACTTTTTTT64050.2542742287015063No Hit
TATCAACGCAGAGTACTTTTTTTTT61740.2451036827483372No Hit
GTCCTACAGTGGACATTTCTAAATT36100.14331459260147347No Hit
CTGTAGGACGTGGAATATGGCAAGA35720.1418060179425106No Hit
GTCCTAAAGTGTGTATTTCTCATTT32150.12763335601488568No Hit
CTTTAGGACGTGAAATATGGCGAGG30890.1226312400404298No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30850.12247244270790739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7600.012.5081074
GGACCGT855.3293E-511.1757226
GTCCTAC19750.011.0341751
GGTATCA20800.011.0261841
TGTAGGA23550.010.9011672
GTAGGAC23200.010.6124813
AAGGCGT1909.094947E-1210.4992986
CTGTAGG23850.010.4939481
TCTATAC2550.010.4381383
GGACGTG18950.010.4267436
CGCCCTA1553.6980055E-910.41390116
AGGACGT19500.010.3836525
AGGACCG1106.0022558E-610.37035755
GGCGTGC1659.331416E-1010.3619148
GTCCTAA10500.010.3320641
GGCGAGG9550.010.24030319
TTAGGAC12000.010.1399053
TAGGACA8450.010.1249054
AGAACCG951.6340167E-410.0064865
CCGTCCA951.647502E-49.9983399
Produced by FastQC (version 0.11.5)