FastQCFastQC Report
Thu 26 May 2016
SRR1512661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899537
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63800.3358713202217172No Hit
GGTATCAACGCAGAGTACTTTTTTT41510.2185269357743492No Hit
TATCAACGCAGAGTACTTTTTTTTT37720.1985747053097676No Hit
GTCCTACAGTGGACATTTCTAAATT29120.1533005148096615No Hit
GTCCTAAAGTGTGTATTTCTCATTT28820.15172118258291362No Hit
CTGTAGGACGTGGAATATGGCAAGA25730.1354540606474104No Hit
CTTTAGGACGTGAAATATGGCGAGG22860.12034511567818895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5200.012.7719284
GGTATCA12950.011.4519331
TCCAACG1105.1477946E-711.19673518
GACCGGG600.00593096111.0695917
TAATACT2800.010.8431064
GTCCTAC15950.010.7757871
TTAGTAC1158.9696005E-710.7249633
GTCTTAA2300.010.6753921
GGCGAGG8800.010.65899619
TCCTACA18300.010.21328452
GTATTAG1459.950054E-810.16002751
TTAGACG750.00267658910.1202334
TTAGGAC11100.010.0857753
GACGTGA9600.010.0812357
GTGTAGA3800.010.0798171
GTCTTAC2251.8189894E-1210.0396131
GTCCTAA9800.010.0217961
GTATCAA26100.010.0095081
GTATAAT2950.09.9878241
GTCTTGG2950.09.9878241
Produced by FastQC (version 0.11.5)